CORE--VIROLOGY CORE LABORATORY

科罗拉多州ACTU在抗逆转录病毒研究方面的成功 治疗有赖于基础病毒学实验室的密切支持。这个 科罗拉多州ACTU的病毒学核心实验室已经实现了这一目的 自1990年8月我分部成立以来，通过了 ACTG病毒学委员会在HIV-1培养和血清p24中的表现 抗原检测。因此，提交了一份提案，以提供病毒学核心 为科罗拉多州ACTU临床核心中心和其他机构提供实验室支持 按照RFA的规定，与ACTU合作。除了血清p24 抗原检测和HIV-1定性培养，我们的实验室可以 进行血浆病毒血症检测、HIV-1微量定量培养、齐多夫定 耐药检测及聚合酶链式反应扩增HIV-1DNA RT基因分型反应。其他技术可用于 量化病毒载量和检测抗逆转录病毒药物敏感性 正在开发或将在它们可用时被采用。

The success of the Colorado ACTU in performing studies of antiretroviral therapy depends on the close support of a basic virology laboratory. The Virology Core Laboratory of the Colorado ACTU has fulfilled this purpose since establishment of our subunit in August, 1990 and is certified by the ACTG Virology Committee in the performance of HIV-1 cultures and serum p24 antigen assays. A proposal is therefore submitted to provide virology core laboratory support to the Colorado ACTU Clinical Core and to other cooperating ACTU's as outlined in the RFA. In addition to serum p24 antigen determinations and qualitative HIV-1 culture, our laboratory can perform plasma viremia assays, quantitative HIV-1 microcultures, zidovudine resistance assays, and amplification of HIV-1 DNA by polymerase chain reaction for genotype analysis of RT. Additional techniques for quantifying viral load and assaying antiretroviral drug susceptibility are being developed or will be adopted as they become available.