海洋真菌次级代谢产物已成为天然产物研究的热点领域，是寻找新型药物先导化合物的重要资源。面对近来研究中越来越多的出现菌株“重复研究”和.活性化合物“重复发现”问题，为了高效的快速发现并分离真菌中有研究价值的活性化合物，本课题以海洋来源真菌为研究对象，基于建立的ITS-HPLC-HRMS-Bioassay相结合的活性产物快速检识方法，开展如下研究：1)以副溶血弧菌抑制活性和种属鉴定结果为指导确定目标菌株。2)运用HPLC-PDA-HRMS分析发酵产物各馏分色谱峰的UV和HRMS信息，根据其活性及数据库检索结果为指导，对活性化合物进行快速的追踪分离和结构解析。该研究会发现一批具有新颖结构且抗菌活性显著的真菌代谢产物，为真菌资源的有效利用提供一定的科学依据。

The investigation of structurally novel and biologically active compounds from marine-derived fungi has become a noticeable research area in natural drug discovery, as an important source for new drug leads. Recently, facing with increasing troubles of strain of "replication of research" and the active compound "duplicate discovery", in order to search for novel bioactive natural products quickly, our group initiated a program of rapid screening for new compounds based on ITS-HPLC-HRMS-Bioassay integrated methods. Here, we will conduct the following research using sponge-associated fungi as materials: 1) to gain the targeted strain based on inhibitory activity against Vibrio parahaemolyticus and species identification. 2) to rapidly separate and elucidate the active compounds from strain according to their activity screening and database search results, followed by HPLC-PDA-HRMS analysis for the UV and HRMS information of the metablite fractions. The approaches used in this study will greatly accelerate the discovery of some new anti-bacteria fungal secondary metabolite, and will provide a scientific basis for the effective use of marine fungi.