DIAGNOSIS OF ENTAMOEBA HISTOLYTICA INFECTION

据信溶组织内阿米巴感染了5亿人 每年。 大约10%的人出现症状， 患上结肠炎和肝脓肿， 每年4万人。 目前的诊断方法是 粪便标本中微生物的显微镜观察。 然而，在这方面， 这种方法需要丰富的经验，是乏味的，并且可能不适合于 如果不在采集标本后几分钟内进行，则不准确。 在 此外，T不能区分非致病性和致病性菌株。 这是一个主要的缺点，因为非致病性菌株不会引起 感染非致病性菌株的人不需要 治疗与抗阿米巴药物，其中有一些苛刻的一面 方面的影响. 以下项目将评估单克隆抗体的使用 基于抗体的ELISA，旨在检测微生物的存在， 粪便标本，并区分非致病性致病菌株。 ELISA是基于使用单克隆抗体， 特别是生物体的半乳糖粘附素。 该项目将 优化ELISA作为诊断试验，并开始分析粪便 标本以及肝活检和血清标本的存在 溶组织肠杆菌抗原的 结果将与显微镜检查结果相关联 结果和酶模分析，这是昂贵和繁琐的， 是目前区分非致病性和致病性的唯一方法 菌株 ELISA检测将扩大规模用于商业用途， 应用程序，应该是有用的诊断辅助工具，并将使 诊断更有效，更具成本效益。

Entamoeba histolytica is believed to infect a half billion people annually. Approximately 10% of these individuals become symptomatic and develop colitis and liver abscess with a mortality rate of more than 40,000 persons each year. The current methods of diagnosis is the microscopic visualization of the organism in fecal specimens. However, this method requires extensive experience, is tedious, and may not be accurate if not performed within minutes of collecting the specimen. In addition, t does not distinguish nonpathogenic and pathogenic strains. This is a major drawback because nonpathogenic strains do not cause disease and persons infected with nonpathogenic strains do not require treatment with antiamebic drugs, which have a number of harsh side effects. The following project will evaluate the use of monoclonal antibody-based ELISAs designed to detect the presence of the organism in fecal specimens and to distinguish nonpathogenic from pathogenic strains. The ELISAs are based on the use of monoclonal antibodies that bind specifically to the galactose adhesin of the organism. The project will optimize the ELISA as a diagnostic test and begin to analyze fecal specimens as well as liver biopsy and serum specimens for the presence of E.histolytica antigen. Results will be correlated with microscopy results and with zymodeme analysis, which is expensive and tedious and is the only current means of distinguishing nonpathogenic from pathogenic strains. The ELISA tests, which will be scaled up for commercial application, should be useful as diagnostic aids and will make the diagnosis more efficient and cost-effective.