CELL ADHESION IN BIOLOGICAL SIGNAL TRANSDUCTION

蛋白酪氨酸磷酸酶对细胞粘附的调节 细胞外基质。 G.B. Schneider 1-2和K. BURRIDGE 2（部门） 的 口腔修复学和细胞生物学2;北卡罗来纳州，查珀尔大学 Hill）。 我的研究包括研究细胞粘附和细胞内信号传导 成纤维细胞和成骨细胞局灶性粘连。 局灶性粘连 连接细胞外基质与细胞外基质的跨膜结构。 细胞骨架 粘着斑有助于细胞的能力， 调节参与粘附、细胞生长的信号通路， 分化 这些调节机制在临床上是相关的， 手术干预后的伤口愈合，正常细胞生长， 分化和肿瘤发生中的基因表达模式。 这些 当人们考虑病因时， 在我们治疗的许多颌面部患者中， 经历了与衰老相关的进行性骨质流失，或者在 骨整合机制发生在愈合和加载过程中， 植入物. 我的论文的总体目标是尝试并确定一种酶， 粘着斑有助于调节这些信号通路。 一 这种潜在的酶被称为蛋白酪氨酸磷酸酶（PTP）。 这种类型 酶的作用被认为是对抗致癌产物的作用 由酪氨酸激酶（PTK）产生。 我试图找出一个 这些磷酸酶通过开发多克隆抗体， 磷酸酶抗原，包括合成肽和GST融合蛋白 proteins. 未来的实验将涉及使用这些抗体， 细胞的免疫荧光标记、生物化学分析， 免疫沉淀和蛋白质印迹，以及显微注射研究， 评估细胞的形态和生理反应。 其他 研究将评价亲和层析研究生成的数据， 以及一种新的PTP测定法。 收集的数据将帮助我们更好地 了解PTP在信号通路调节中的作用 参与各种细胞内粘附、分化和有丝分裂 类型

Protein Tyrosine Phosphatase Regulation of Cellular Adhesion to Extracellular Matrices. G.B. SCHNEIDER 1-2 and K. BURRIDGE2 (Depts. of Prosthodonticsl and Cell Biology2; University of North Carolina,Chapel Hill). My research involves studying cellular adhesion and intracellular signalling in fibroblast and osteoblast focal adhesions. Focal adhesions are transmembrane structures that link the extracellular matrix with the cytoskeleton. The focal adhesion contributes to the cells ability to regulate signalling pathways involved in adhesion, cell growth, and differentiation. These regulatory mechanisms are clinically relevant during wound healing following surgical interventions, in normal cell growth and differentiation, and in patterns of gene expression in tumorigenesis. These signalling pathways are important when one considers the etiological factors of many of the maxillofacial patients we treat, in patients who have experienced progressive bone loss associated with aging, or in the osseointegrative mechanisms occurring during healing and loading of implants. The overall goal of my thesis is to try and identify an enzyme within the focal adhesion that helps to regulate these signalling pathways. One potential enzyme is called a protein tyrosine phosphatase (PTP). This type of enzyme is thought to antagonizes the effects of oncogenic products generated by tyrosine kinases (PTK). I have attempted to identify one of these phosphatases by developing polyclonal antibodies to potential phosphatase antigens, including both synthetic peptides and GST-fusion proteins. Future experiments will involve using these antibodies for immunofluorescent labelling of cells, biochemical analysis such as immunoprecipitations and Western blots, and microinjection studies to evaluate the morphological and physiological response of the cell. Other studies will evaluate data generated by affinity chromatography studies, as well as a novel PTP assay. The data gathered will help us to better understand the role of PTP's in the regulation of signalling pathways involved in adhesion, differentiation, and mitogenesis within various cell types.