IMAGING THE RESPONDING SI NEURONAL POPULATION

所提出的实验重点研究刺激诱发的模式 活动在躯体感觉皮层（SI），特别是，对 由重复的皮肤刺激诱发的激活模式。 在01 年，动物实验采用C14-2DG代谢作图法（2DG） 本发明公开了一种用于内皮层成像的红外光学记录方法， 将执行信号（IR成像）。 目标是获得和 比较IR和2DG图像的皮层反应相同的刺激 条件 在02-05年，红外光学记录技术将是 用于表征刺激诱发的SI活动的影响 模式（i）NMDA受体阻滞，（ii）不同频率的 振动触觉刺激，和（iii）不同的时空模式， 皮肤刺激。 预计拟议的研究将 提供有关刺激后果的新颖而有用的信息- 诱发多个空间分布的神经元之间的相互作用 由皮肤刺激激活的群体。 的资料 获得的应该填补目前已知的主要空白， 躯体感觉皮层对皮肤刺激反应-可归因于间隙 主要是由于方法的不足，至少到目前为止， 已经被实验所强调。 此外，调查结果应 为正在进行的刺激建模和模拟研究提供方向- 定向躯体感觉皮层网络动力学和潜在的 神经机制

The proposed experiments focus on studies of patterns of stimulus evoked activity in somatosensory cortex (SI), and in particular, on the activation patterns evoked by repetitive skin stimulation. In the 01 year, animal experiments using the C14-2DG metabolic mapping method (2DG) and the infrared optical recording method for imaging intrinsic cortical signals (IR imaging) will be performed. The goal is to obtain and compare IR and 2DG images of the cortical response to the same stimulus conditions. In years 02-05, the IR optical recording technique will be employed to characterize the effects on stimulus evoked SI activity patterns of (i) NMDA receptor block, (ii) different frequencies of vibrotactile stimulation, and (iii) differing spatiotemporal patterns of skin stimulation. It is anticipated that the proposed studies will provide novel and useful information about the consequences of stimulus- evoked interactions among the multiple and spatially distributed neuronal populations activated by cutaneous stimuli. The information to be obtained should fill major gaps in what is currently known about the somatosensory cortical response to skin stimulation-gaps attributable principally to inadequacies of the methodologies which, at least to date, have been emphasized by experiments. In addition, the findings should provide direction to ongoing modeling and simulation studies of stimulus- directed somatosensory cortical network dynamics and the underlying neural mechanisms.