CORTICAL MODULATION OF SOMATOSENSATION

描述：以前的研究已经确定了一种复杂的模式， 皮层通路的感觉调节。 的重要领域 调制是背柱核。 背柱神经元 核可以接受来自谷氨酰胺能或谷氨酸能的输入 皮层突触 这种输入可能发生在中间神经元上， 中继神经元 拟议研究的一个领域是确定在 EM水平，使用多种标记技术，神经化学物质 包含在背柱已识别亚群的传入神经中 核神经元。 这一初步工作之后将进行鉴定 背柱核表达的氨基酸受体 神经元 将使用免疫细胞化学定位，以及 使用寡核苷酸探针的原位杂交。 Postembedding 免疫金技术有望发展到定位受体 在EM级别。 最后，该提案计划继续分析 没有神经元，并确认所有确实是interneurons，而不是 神经元与其他目标区域。 再次使用双标签 方法学，NO与甘氨酸而不是GABA共定位， 将探索背柱核神经元。

DESCRIPTION: Previous research has identified a complex pattern of sensory modulation by cortical pathways. An important area of modulation is the dorsal column nuclei. Neurons in the dorsal column nuclei may receive input from either glutamergic or aspartergic cortical synapses. This input may occur on either interneurons or relay neurons. One area of the proposed research is to determine at the EM level, using multiple label techniques, the neurochemicals contained in afferents to identified subpopulations of dorsal column nuclei neurons. This initial work will be followed by identification of amino acid receptors that are expressed by dorsal column nuclei neurons. Immunocytochemical localization will be used, as well as in situ hybridization using oligonucleotide probes. Postembedding immunogold techniques are hoped to be developed to localize receptors at the EM level. Lastly, the proposal plans to continue analysis of NO neurons and confirm that all are indeed interneurons rather than neurons with other target areas. Again, using double label methodology, colocalizaton of NO with glycine rather than GABA in dorsal column nuclei neurons will be explored.