CORTICAL MODULATION OF SOMATOSENSATION

描述：以前的研究发现了一种复杂的 大脑皮层通路的感觉调节。一个重要的领域 调制是背侧柱核。背柱中的神经元 核团可能接受谷氨酰胺能或天冬氨酸氨基转移酶能的输入 大脑皮层突触。这种输入可能发生在中间神经元或 中继神经元。拟议研究的一个领域是确定 EM水平，使用多重标记技术，神经化学物质 包含在已识别的背柱亚群的传入中 核神经元。这项初步工作之后将进行身份识别 由背柱核表达的氨基酸受体 神经元。将使用免疫细胞化学定位，以及在 使用寡核苷酸探针的原位杂交。嵌入后 人们希望开发免疫金技术来定位受体。 在新兴市场层面。最后，提案计划继续分析 没有神经元，并确认所有确实是中间神经元，而不是 神经元与其他靶区。同样，使用双重标签 方法学中，NO与甘氨酸而不是GABA共定位 本课程将探讨背柱核神经元。

DESCRIPTION: Previous research has identified a complex pattern of sensory modulation by cortical pathways. An important area of modulation is the dorsal column nuclei. Neurons in the dorsal column nuclei may receive input from either glutamergic or aspartergic cortical synapses. This input may occur on either interneurons or relay neurons. One area of the proposed research is to determine at the EM level, using multiple label techniques, the neurochemicals contained in afferents to identified subpopulations of dorsal column nuclei neurons. This initial work will be followed by identification of amino acid receptors that are expressed by dorsal column nuclei neurons. Immunocytochemical localization will be used, as well as in situ hybridization using oligonucleotide probes. Postembedding immunogold techniques are hoped to be developed to localize receptors at the EM level. Lastly, the proposal plans to continue analysis of NO neurons and confirm that all are indeed interneurons rather than neurons with other target areas. Again, using double label methodology, colocalizaton of NO with glycine rather than GABA in dorsal column nuclei neurons will be explored.