RETROGRADE TRANSPORT/NUCLEAR IMPORT PATHWAY IN NEURONS

描述：关于再生和逆行运输的研究 失活神经元鉴定了一组逆行转运蛋白 其中包含核输入信号。 该信号肽序列 是同源的核输入序列中发现的各种 其他蛋白质，如S40大T抗原和外源性 带有这种序列的蛋白质会受到两种逆行 运输和核输入时引入轴突。 蛋白 现在已经获得并鉴定了含有这些序列的DNA， 大鼠 这些蛋白质被认为代表了 一种难以捉摸的神经损伤信号，被认为是介导细胞体 神经损伤后的反应。 本应用中的实验 旨在表征含有这些的内源性蛋白质 序列及其相关的多肽。这些生物学 将探索Astrasia和大鼠神经中的蛋白质， 提供神经损伤后细胞体反应的见解， 识别潜在的分子机制。

DESCRIPTION: Studies on regeneration and retrograde transport in Aplysia neurons identified a set of retrogradely transported proteins which contain a nuclear import signal. This signal peptide sequence is homologous to the nuclear import sequences found in a variety of other proteins, such as the S40 large T-antigen and exogenous proteins with this sequence attached are subject to both retrograde transport and nuclear import when introduced into the axon. Proteins containing such sequences have now been obtained and identified in rat. These proteins are thought to represent prototypes for the elusive nerve injury signal which is thought to mediate the cell body response following nerve injury. The experiments in this application are intended to characterize endogenous proteins containing these sequences and their associated polypeptides.The biology of these proteins in Aplysia and rat nerve will be explored in the hopes of providing insights into the cell body response after nerve injury and identifying the underlying molecular mechanisms.