QUANTITATIVE STUDY OF IFN-G AND BRAIN TUMOR SPREAD

传统疗法的进展在成像方面越来越成功 以及对脑瘤肿块进行消毒。需要新的方法来攻击 残留的播散性或浸润性肿瘤。这导致了 免疫疗法作为一种辅助治疗方式重新引起了人们的兴趣。 假设 1.在荷瘤小鼠脑内注射干扰素-g可以 激活抗原提呈细胞，招募准备好的辅助性T细胞。 2.如果T细胞已经被肿瘤抗原激活，结果 免疫级联可导致播散性脑瘤的破坏 细胞。 具体目标 一、我们将完成干扰素-g诱导的无肿瘤细胞变化的分析 老鼠。 答：我们将确定不同药物的剂量-反应曲线 干扰素-g介导的效应，我们以前在未免疫的动物中定义过。 B.我们将比较干扰素-g介导的大鼠在 被神经或肿瘤抗原激活的。 二、干扰素-g治疗与免疫协同作用控制 肿瘤细胞随脑部扩散？ 答：我们将确定单独使用干扰素-g治疗对肿瘤扩散的影响。 B.我们将确定[干扰素-g治疗+免疫]对 肿瘤扩散。 C.我们将确定[干扰素-g治疗+肿瘤免疫 抗原]会导致自身免疫性疾病。 方法：研究方法。这些研究将在活体的大鼠模型中进行。 立体定向手术、组织化学、免疫细胞化学和 将使用计算机辅助图像分析来建立和分析 肿瘤模型。

Advances in conventional therapies are increasingly successful in imaging and sterilizing a brain tumor mass. New approaches are needed to attack the disseminated or infiltrating tumor that remains. This has lead to renewed interest in immunotherapy as an adjunctive mode of treatment. HYPOTHESES 1. In the tumor-bearing brain, intracerebral injection of IFN-g can activate antigen-presenting cells and recruit primed helper T cells. 2 . If the T cells have been primed to tumor antigen, the resulting immune cascade can lead to the destruction of disseminated brain tumor cells. SPECIFIC AIMS I. WE WILL COMPLETE THE ANALYSIS OF IFN-g-INDUCED CHANGES IN TUMOR-FREE RATS. A. We will determine the dose-response curve for the different IFN-g-mediated effects we have previously defined in unimmunized animals. B. We will compare the IFN-g-mediated changes in rats that have been primed to neural or tumor antigens. II. DO IFN-g TREATMENT AND IMMUNIZATION WORK SYNERGISTICALLY TO CONTROL THE SPREAD OF TUMOR CELLS WITH THE BRAIN? A. We will define the effects of IFN-g treatment alone on tumor spread. B. We will define the effects of [IFN-g treatment + immunization] on tumor spread. C. We will determine whether [IFN-g treatment + immunization to tumor antigen] causes autoimmune disease. METHODS. These studies will be performed in a rat model, in vivo. Stereotactic surgery, histochemistry, immunocytochemistry, and computer-assisted image analysis will be used to establish and analyze the tumor model.