MAPPING AND CHARACTERIZATION OF AGE MUTANTS

这一研究方向的长期目标是 了解导致衰老的分子和生理过程， 线虫秀丽隐杆线虫我发现了三个新的等位基因 age-1是一种延长寿命的基因。我们建议确定 这些延长寿命作用的分子和生理基础， 直接检验寿命延长的假设 从增强介导抵抗有毒物质的能力的过程， 应力我的目标是（A）完成回交，互补测试， 新分离的Age突变体的作图;（B）应力测试， 抗性表型（耐热性、抗UV性和抗H2 O2性） （C）如果突变体显示出增加的耐热性， 确定HSP-16是否更容易诱导，就像年龄一样， 1;和（D）评估行为、发育和其他生活史特征 新时代的变种人

The standing long-term goal of this research direction has been to understand the molecular and physiological processes that lead to aging in the nematode Caenorhabditis elegans. I have found three new alleles of age-1 which is a gene that extends life span. We propose to identify the molecular and physiological bases of these life-prolongation effects and to directly test the hypothesis that prolongation of life span results from the enhancement of processes mediating the ability to withstand toxic stress. My aims are to (A) finish backcrosses, complemenation tests and mapping of the newly isolated Age mutants; (B) test for the stress- resistant phenotypes (thermotolerance, UV resistance, and H2O2 resistance) of each of these strains; (C) if mutants show increased thermotolerance, determine whether hsp-16 is more readily induced, as is the case for age- 1; and (D) assess behavior, development and other life history traits on the new Age mutants.