NOVEL NEURONAL CALCIUM CHANNEL

我们最近克隆了一种新的钙通道α 1亚基， 名为Alpha1e。 该频道的文字稿广泛分发于 神经系统 然而，alpha1e的表达产生Ca电流， 在很多方面都很神秘 的生物物理和 电流的药理学特征与那些 一般都有报道，但也有一些例外。 我们现在 希望进一步调查情况，以确定 正常细胞中基于α 1e的电流的特性及其 生理功能。 为了做到这一点，我们将采取各种各样的 该方法将包括以下类型的实验。第一章 制备用于检查α 1e的抗体 蛋白质的分布及其表达。2)表达不同 α 1e剪接变体与不同辅助 亚基，以提供关于基于α 1的Ca电流如何 通常会出现。3)研究α 1电流是否可以 由不同的第二信使和受体调节。4)去除 α 1 e转录物来自它们通常发生的细胞， 检查这对细胞功能的影响。 5)考试 alpha1e不同形式的分布。 这些实验 将结合生物物理学和分子生物学 范例 希望这些研究能为我们提供重要的 关于这种新的神经元钙通道的功能的信息。

We have recently cloned a novel Ca channel alpha1 subunit which we have named alpha1e. The transcript for this channel is widely distributed in the nervous system. However, expression of alpha1e produces Ca currents that are mysterious in several respects. The biophysical and pharmacological profile of the currents are not really similar to those that have generally been reported - with a number of exceptions. We now wish to investigate the situation further with a view to establishing the characteristics of alpha1e based currents in normal cells and their physiological functions. In order to do this we shall take a varied approach which will include the following types of experiments. 1) Preparation of antibodies against alpha1e to be used for examining the distribution of the protein and its expression. 2) Expression of different splice variants of alpha1e in combination with different ancillary subunits in order to provide information on how alpha1e based Ca currents may normally appear. 3) Investigations of whether alpha1e currents can be regulated by diverse second messengers and receptors. 4) Removal of alpha1e transcripts from cells in which they normally occur and examination of the consequences of this for cell function. 5) Examination of the distribution of the different forms of alpha1e. These experiments will utilize a combination of biophysical and molecular biological paradigms. It is hoped that these studies will provide us with important information about the functions of this novel neuronal Ca channel.