COMPUTER MODELING OF CILIARY MECHANISMS

纤毛是一种复杂的运动细胞器，广泛存在于 真核细胞，在原生动物和无脊椎动物后生动物以及 脊椎动物。该细胞器的结构和运动机制 在整个进化过程中都是保守的，但分子基础的细节 运动的问题仍未解决。这个项目的总体目标是建立 高分辨率的纤毛轴丝功能计算机模型。 这一动态模型将结合结构、机械和 几种临界轴线的力生成性质 组件，以说明弯曲是如何形成的，以及弯曲波是如何形成的 沿着完整的轴丝以无法获得的分辨率传播 之前。结构和其他特征的适当测量 在电子显微照相中定义缺失的元素，例如 二元组间连接的性质，将与分析 系统在不同复杂程度下的物理属性， 以允许计算随时间变化的 以双人滑行开头的模型。然后，约束将是 介绍了生成轴丝分裂然后弯曲的模型。 使用适当的软件成功完成工作模型 包装将成为细胞器结构生物学的有力工具 被未来的调查人员使用。

The cilium is a complex motile cell organelle that occurs widely in eukaryotic cells, in protozoa and invertebrate metazoa as well as vertebrates. The structure and mechanism of motility of this organelle are conserved throughout evolution, but details of the molecular basis of motion remains unsolved. The overall aim of this project is to build a functional computer model of the ciliary axoneme at high resolution. This dynamic model would incorporate the structural, mechanical and force-generating properties of each of several critical axonemal components to illustrate how a bend is formed and how a bending wave is propagated along a complete axoneme at a level of resolution unobtainable previously. Appropriate measurements of structural and other features in electron micrographs to define missing elements, such as the properties of the interdoublet links, would be combined with analysis of the physical properties of the system at various levels of complexity, to permit the computation of time-dependent structural changes in the model beginning with doublet sliding. Constraints will then be introduced to generate a model of axonemal splitting and then of bending. The successful completion of the working model with appropriate software packing will be a powerful tool of organelle structural biology to be used by future investigators.