MOLECULAR DIAGNOSTIC TESTS FOR SIMIAN HERPESVIRUS

猴子，特别是恒河猴，是一种重要的资源， 生物医学研究 猴疱疹病毒（B病毒），一种天然的 猕猴，对从事生物医学工作的人员来说是一个非常真实的危险。 与这些动物。 检测受感染猴子的灵敏测定法 需要识别对人员构成风险的动物， 鉴定无B病毒动物用于建立无B病毒 繁殖殖民地 H. cercopithicus 1（SA 8）在狒狒中引起问题 繁殖殖民地 SA 8向人类传播的发生率为 未知可区分B病毒、SA 8的灵敏度检测 两种人类单纯疱疹病毒（HSV）是 准确和快速地鉴定人猴疱疹病毒感染。 灵长类疱疹病毒广泛的抗原相关性已经被证实， 是实现这一目标的主要障碍。 根据生成的数据 从我们实验室的分子研究中，我们将开发出敏感的 快速检测和鉴别灵长类病毒的ELISA试验 以及抗B病毒和SA 8的抗体。 这些测试将基于特定的 病毒糖蛋白 将开发一种用于检测灵长类疱疹病毒的PCR方法， 临床样本。 使用位于该基因保守区域的引物组， 糖蛋白gB基因，该基因的病毒特异性区域将被 从四种灵长类疱疹病毒中的任何一种病毒中扩增出来。 后续 对PCR产物的分析将能够确定哪种病毒 (HSV1、HSV 2、B病毒或SA 8）。 ELISAs 还将开发用于疑似人类和 猴B病毒和/或SA 8病例。 所有的gG糖蛋白同源物 四种病毒是抗原性病毒特异性的。 SA 8和SA 8的gG基因 BV将被克隆、测序并在哺乳动物细胞中稳定表达。 这些细胞系和抗gG抗体将用于开发 灵敏的病毒特异性ELISA检测BV和SA 8抗体。 该项目将基础分子研究成果应用于 B病毒和SA 8的检测和诊断是临床上的重要问题。 开发的检测方法将允许快速，灵敏和可靠 灵长类疱疹病毒感染的鉴别诊断。 这将 使灵长类动物群体管理者能够更有效地管理灵长类动物， 设施和减少人畜共患疱疹造成的生命损失 感染.

Monkeys, particularly rhesus macaques, are a vital resource for biomedical research. Herpesvirus simiae (B virus), a natural pathogen of macaques, presents a very real danger for biomedical personnel who work with these animals. Sensitive assays for detection of infected monkeys are needed to identify animals which pose a risk to personnel and to identify B virus-free animals for use in establishment of B virus-free breeding colonies. H. cercopithicus 1 (SA8) causes problems in baboon breeding colonies. The incidence of transmission of SA8 to humans is unknown. Sensitive assays which can differentiate between B virus, SA8 and the two human herpes simplex viruses (HSVs) are necessary for accurate and rapid identification of human simian herpesvirus infections. The extensive antigenic relatedness of the primate herpesviruses has been a major impediment to achieving this goal. Building on data generated from molecular research in our laboratory, we will develop sensitive ELISA tests for rapid detection and differentiation of primate viruses and antibody to B virus and SA8. These tests will be based on specific viral glycoproteins. A PCR assay will be developed for detection of primate herpesviruses in clinical samples. Using a primer set located in conserved regions of the glycoprotein gB gene, a virus-specific region of the gene will be amplified from any of the four primate herpesviruses. Subsequent analysis of the PCR product will enable determination of which virus (HSV1, HSV2, B virus or SA8) is present in the clinical samples. ELISAs will also be developed for serological detection of suspected human and simian B virus and\or SA8 cases. The gG glycoprotein homologs of all four viruses are antigenically virus-specific. The gG genes of SA8 and BV will be cloned, sequenced and stably expressed in mammalian cells. These cell lines and anti-gG antibodies will be used to develop sensitive, virus-specific ELISAs for detection of antibody to BV and SA8. This project applies results of basic molecular research to the clinically important problem of B virus and SA8 detection and diagnosis. The assays developed will permit rapid, sensitive and reliable differential diagnosis of primate herpesvirus infections. This will enable primate colony managers to more effectively operate primate facilities and reduce loss of human life due to zoonotic herpes infections.