CELL ADHESION AND MATRIX IN VERTEBRATE DEVELOPMENT

拟议的研究计划的主要目标是建立 整合素和细胞外基质分子对细胞外基质的功能贡献 帮助形成早期两栖动物的形态发生过程 胚胎。为了更好地了解整合素的功能和表达 在胚胎发育中，非洲爪哇整合素α亚基特异的cDNA和 将会获得抗体。免疫细胞化学和原位观察 将利用杂交方法来确定空间 以及胚胎中整合素mRNA和蛋白表达的时间模式。 然后将使用几种方法来分析 特异性整合素受体。整合素转录本将被注入 卵和特定的卵裂球与人卵母细胞的黏附、迁移和命运 随着发育的进行而确定的后代。粘附性与迁移性 控制基因和显性基因在胚胎细胞中的过表达行为 我们将对负整合素结构进行比较。整合素的变化 将使用以下方法研究诱导后介导的细胞黏附 来自注入转录本的胚胎的细胞和外植体 已知可诱导中胚层的编码多肽。整合素的调控 整合素配体结合活性的表达和/或激活 激活素诱导的组织也将在信使核糖核酸和蛋白质上进行研究 级别。

The primary goal of the proposed research program is to establish the functional contribution of integrins and ECM molecules to the morphogenetic processes that help shape the early the early amphibian embryo. In order to better understand integrin function and expression in embryogenesis, Xenopus integrin alpha subunit specific cDNAs and antibodies will be obtained. Immunocytochemistry and in situ hybridization methods will be utilized in order to determine the spatial and temporal patterns of integrin mRNA and protein expression in embryos. Several approaches will then be employed to analyze the functions of specific integrin receptors. Integrin transcripts will be injected into eggs and specific blastomeres and the adhesion, migration and fate of the progeny determined as development proceeds. The adhesive and migratory behaviors of embryonic cells over-expressing control and dominant- negative integrin constructs will be compared. Changes in integrin mediated cell adhesion in response to induction will be studied using cells and explants derived from embryos injected with transcripts encoding peptides known to induce mesoderm. The control of integrin expression and/or the activation of integrin ligand binding activity in activin-induced tissues will also be investigated at the mRNA and protein levels.