ASTROCYTE-EXPRESSED GENES INFLUENCING NEURITE TARGETING

建议的研究将有助于对轴突的理解 靶向神经系统(NS)。对这一过程的了解可能 帮助设计HeNS的体细胞基因治疗，特别是在一些 退行性疾病和对NS的机械损伤。 分子生物学技术(cDNA克隆。MRNA指纹图谱， 测序和序列分析)将与 星形胶质细胞培养寻找人群中区域表达的基因 星形胶质细胞。 新生大鼠脑不同部位星形胶质细胞的培养、纯化 将被用作信使核糖核酸的来源，用信使核糖核酸进行分析 利用指纹图谱技术寻找胎儿组织差异表达的基因克隆 大鼠脑c DNA文库。选定的cDNAs将被测序，并 将搜索序列以寻找有关其功能的线索。 如果时间允许，将尝试验证功能重要性 反义mRNA实验中差异表达的cDNAs 通过星形胶质细胞和神经元的共培养。

The proposed research should contribute to the understanding of axonal targeting in the nervous system (NS). Knowledge about this process could help in designing of somatic gene therapy in he NS, especially in some degenerative disorders and in mechanical damage to the NS. Molecular biology techniques (cDNA cloning. mRNA fingerprinting, sequencing, and sequence analysis) are to be used in conjunction with astrocyte culturing to find genes regionally expressed in population of astrocytes. Culture, purified astrocytes from various parts of neonatal rat brain will be used as a source of mRNA which will be analyzed by mRNA fingerprinting to find the differentially expressed cDNA clones in fetal rat brain cDNA libraries. The selected cDNA's will be sequenced and the sequences will be searched for clues about their function. If time allows it will be attempted to verify the functional importance of the differentially expressed cDNA's in anti-sense mRNA experiments with co-cultures of astrocytes and neurons.