PEPTIDERGIC MODULATION OF SYMPATHETIC COTRANSMISSION

研究期间提出的研究将为我提供至关重要的 交感神经系统活动研究的经验。 因此，我的主要 这一时期的目标是：（一）研究 心血管肽ET、ANP和BNP对突触前和突触后 外周交感神经共传递的机制， 离体组织和缺乏中枢反射的生物体 在体内以及研究内皮的参与， EDRF/NO、cGMP、IP 3和Ca++在这些效应中的作用;（ii）了解基本的 外周交感神经研究的必要技术 共同传播，特别是与 a）发射机（ATP和 NE）释放测量，B）分离的血液中的收缩反应 血管， c）细胞内Ca++和环核苷酸信号传导，以及 d）倾斜动物的血流动力学（见项目21，研究计划）。 韦斯特福尔教授的实验室是研究 内源性肽对交感神经传递的影响。 大卫教授 韦斯特福尔和他的团队在开发 交感神经传递涉及释放的概念， 共递质ATP和NE。 此外，他的团队最近还开发了 这一概念，即连接前的嘌呤受体， 神经递质释放，可能存在几个亚型。 实验室 也以其开发新方法的能力而闻名 神经传递的问题。 例如，他的实验室是 世界上只有少数几个地方可以释放ATP和NE， 同时评价。 有机会在这样的环境中学习和工作 环境，特别是在一个地方，研究兴趣是如此 与我自己的一致，无疑将促进我的发展， 科学生涯。

The proposed research during the fellowship will provide me with vital experience of sympathetic nervous system activity studies. Thus, my main goals for this period are: (i) To study the modulating effects of cardiovascular peptides ET, ANP, and BNP on the pre- and postsynaptic mechanisms of peripheral sympathetic cotransmission at the levels of isolated tissues in vitro and of an organism devoided of central reflexes in vivo as well as to study the participation of the endothelium, the EDRF/NO, cGMP, IP3, and Ca++ in these effects; (ii) To learn the basic techniques necessary for studies on peripheral sympathetic cotransmission, particularly those related to a) transmitter (ATP and NE) release measurement, b) contractile responses in isolated blood vessels, c) intracellular Ca++ and cyclic nucleotide signaling and d) hemodynamics of pitched animals (see Item 21, Research plan). Professor Westfall's laboratory is an ideal place to study the role of endogenous peptides on sympathetic neurotransmission. Professor David Westfall and his group have played a fundamental role in developing the concept that sympathetic neurotransmission involves the release of the cotransmitters ATP and NE. In addition his group has recently developed the concept that prejunctional purinoceptors, which modulate neurotransmitter release, may exist as several subtype. The laboratory is also well known for its ability to develop new methodologies to apply to issues of neurotransmission. For example, his laboratory is one of the few in the world where the release of ATP and NE can be simultaneously evaluated. The opportunity to learn and work in such an environment particularly in a place where the research interests are so congruent with my own, will no doubt enhance the development of my scientific career.