REAGENT PRODUCTION SUPPORT FOR RESEARCH ON AIDS

这份合同的目的是支持美国国家科学研究院 过敏和传染病(NIAID)的使命是刺激 发现后天获得者改进治疗方法的研究 免疫缺陷综合征(艾滋病)和相关的机会性感染。 NIAID需要生产最先进的试剂来支持 艾滋病研究人员的实验室研究工作。的发展。 有效对抗艾滋病毒和艾滋病的抗病毒和抗感染药物和疫苗 相关的机会主义微生物因缺乏 标准化的、可免费获得的试剂。这样的试剂是必要的 用于在体外生产特定的药物靶点，从而有效地 候选合成化合物的筛选可以快速进行。 由于机会主义的传播存在技术上的困难 病原体体外、分子试剂亟待开发 改良的监测病人体内负荷的方法 治疗。 承包商将从以下公司获得试剂和起始材料 项目官员，用于修改、放大、生产和 向NIAID艾滋病研究和研究中心运送分子生物学试剂 用于分发给NIAID的参考试剂计划-支持 调查人员。这项活动的目标是准备、评估、 扩增cDNA库和基因组文库；制备表达载体； 生产基因产品；制备、生产和提纯可溶性蛋白质； 制备和生产多克隆和单克隆抗体；并检测， 评估、描述并将生成的试剂运送到NIAID和 研究机会性感染的科学调查人员。产品 根据本合同制作的产品将用于支持研究 科学界的活动。NIAID需要访问 大量已知的材料，以支持 国家合作药物发现小组，艾滋病临床研究 小组、艾滋病基础研究卓越计划和其他 由NIAID发起的特定计划。

The purpose of this contract is to support the National Institute of Allergy and Infectious Diseases (NIAID) in its mission to stimulate research towards discovery of improved therapy for the acquired immunodeficiency syndrome (AIDS) and associated opportunistic infections. The NIAID requires production of state-of-the-art reagents to support laboratory research efforts of AIDS investigators. The development of antiviral and anti-infective drugs and vaccines effective against HIV and associated opportunistic microorganisms has been hampered by the absence of standardized, freely available reagents. Such reagents are necessary for the production of specific drug targets in vitro so that efficient screening of candidate synthetic compounds can proceed expeditiously. Because of technical difficulties in the propagation of opportunistic pathogens in vitro, molecular reagents are urgently needed to develop improved methods to monitor organism load in patients undergoing treatment. The contractor will receive reagents and starting materials from the Project Officer for the purpose of modifying, amplifying, producing, and shipping molecular biology reagents to the NIAID AIDS Research and Reference Reagent Program for distribution to NIAID-supported investigators. It is the objective of this activity to prepare, evaluate, and amplify cDNA and genomic libraries; prepare expression vectors and produce gene products; to prepare, produce, and purify soluble proteins; to prepare and produce polyclonal and monoclonal antibodies; and to assay, evaluate, describe, and ship the resulting reagents to the NIAID and to scientific investigators studying opportunistic infections. The products produced under this contract will be used to support the research activities of the scientific community. The NIAID needs access to significant quantities of the noted materials to support the efforts of the National Cooperative Drug Discovery Groups, the AIDS Clinical Study Groups, the Programs of Excellence in Basic Research on AIDS, and other specific programs initiated by NIAID.