Expansion and modification of animal housing and support spaces to increase production of the NIH U42 supported pigtailed macaque colony at Johns Hopkins University

项目摘要 在面对有充分记录的非人灵长类动物持续短缺的情况下， 研究和证据表明，它们可能是COVID-19的独特模型，因此， 对猪尾猕猴的需求仍然强劲，并继续攀升。自从NIH成立以来， 在2006年的支持下，JHU猪尾猕猴群为生物医学提供了313只动物 研究，其中绝大多数与艾滋病毒/艾滋病有关。其中172只动物（55%） 去JHU以外的机构的NIH资助的研究人员，我们已经建立了我们的殖民地， 作为这种珍贵动物模型的重要国家资源。为了满足日益增长的 猪尾猕猴在生物医学研究中，我们已经制定了一项计划，以显着增加 我们可以在JHU研究农场饲养的动物数量，并进一步完善我们的 行为管理这个繁殖群体，以提高生产力。首先，我们建议 在现有的12号楼的基础上扩建一座建筑，显著扩大动物舍， 支持空间。这个新的建筑将包括动物住房空间和创新的笼子 6个后宫的120只额外动物。此外，还将提供专门的支持 空间，包括手术室，放射室，用于康复或预处理的弹性保持空间， 销售动物和笼清洗区，沿着人员空间，如工作人员储物柜 带连接淋浴间、浴室、洗衣房和PPE穿戴/脱下区的房间。二是 建议安装地下光纤电缆和其他网络基础设施， JHU研究农场设施到高速互联网。这个项目将使我们能够充分利用 即将安装的JHU资助的升级1）安全和2）电子动物健康， 行为记录。如果这项提议成功，我们将更好地为NIH资助的研究服务 社区，依赖于灵长类动物模型在两个关键方面：1）我们将增加数量， 动物提供给研究人员和2）我们将评估新的畜牧业和行为 管理功能设计到新的建设，并分享我们的研究结果与NIH资助的 非人类灵长类研究人员和其他国家育种中心。

Project Summary In the face of well-documented ongoing shortages of nonhuman primates for biomedical research and evidence that they may be a unique model for COVID-19, it is no surprise that the demand for pigtail macaques remains strong and continues to climb. Since the onset of NIH- support in 2006, the JHU pigtail macaque colony has provided 313 animals for biomedical research, the vast majority of which was HIV/AIDS related. With 172 of those animals (55%) going to NIH funded investigators at institutions outside of JHU, we have established our colony as an important national resource of this valuable animal model. To meet the growing need for pigtail macaques in biomedical research, we have developed a plan to significantly increase the number of animals that we can house at the JHU Research Farm and further refine our behavioral management of this breeding colony to improve productivity. First, we propose to construct an addition to the existing Building 12, significantly expanding animal housing and support spaces. This new construction will include animal housing space and innovative caging for 120 additional animals across 6 harems. This addition will also house dedicated support spaces, including an operating room, radiology room, flex holding space for convalescing or pre- sale animals, and a cage washing area, along with personnel spaces such as a staff locker room with connected showers, bathroom, laundry, and PPE donning/doffing area. Second, we propose to install underground fiber optic cable and other network infrastructure to connect the JHU Research Farm facilities to high-speed internet. This project will allow us to fully leverage the soon-to-be-installed JHU-funded upgrades to 1) security and 2) electronic animal health and behavior records. If this proposal is successful, we will better serve the NIH-funded research community that relies on primate models in two key ways: 1) we will increase the number of animals available to researchers and 2) we will evaluate the novel husbandry and behavioral management features designed into the new building and share our findings with NIH-funded nonhuman primate researchers and other national breeding centers.