REAGENT PRODUCTION SUPPORT FOR RESEARCH ON AIDS

该合同的目的是支持国家研究所 过敏和传染病（NIAID）在其使命，以刺激 为发现获得性免疫缺陷综合征的改进疗法而进行的研究 免疫缺陷综合征（艾滋病）和相关的机会性感染。 NIAID需要生产最先进的试剂，以支持 艾滋病研究人员的实验室研究工作。 的发展 有效对抗HIV的抗病毒和抗感染药物和疫苗， 相关的机会性微生物由于缺乏 标准化的，免费提供的试剂 这些试剂是必需的 用于在体外产生特异性药物靶点， 候选合成化合物的筛选可以迅速进行。 由于传播机会主义的技术困难， 病原体的体外分子试剂亟待开发 监测接受手术的患者的生物负荷的改进方法 治疗 承包商将从供应商处获得试剂和起始材料。 项目官员的目的是修改，放大，生产， 将分子生物学试剂运送到NIAID艾滋病研究所， 分发给NIAID支持的参考试剂计划 investigators. 这项活动的目的是编写、评价、 并扩增cDNA和基因组文库;制备表达载体， 生产基因产物;制备、生产和纯化可溶性蛋白质; 制备和生产多克隆和单克隆抗体;以及测定， 评估、描述并将所得试剂运送到NIAID， 研究机会性感染的科学研究者 产品 根据该合同生产的产品将用于支持研究 科学界的活动。 NIAID需要访问 大量上述材料，以支持 国家合作药物发现小组，艾滋病临床研究 小组、艾滋病基础研究卓越计划和其他 由NIAID发起的具体方案。

The purpose of this contract is to support the National Institute of Allergy and Infectious Diseases (NIAID) in its mission to stimulate research towards discovery of improved therapy for the acquired immunodeficiency syndrome (AIDS) and associated opportunistic infections. The NIAID requires production of state-of-the-art reagents to support laboratory research efforts of AIDS investigators. The development of antiviral and anti-infective drugs and vaccines effective against HIV and associated opportunistic microorganisms has been hampered by the absence of standardized, freely available reagents. Such reagents are necessary for the production of specific drug targets in vitro so that efficient screening of candidate synthetic compounds can proceed expeditiously. Because of technical difficulties in the propagation of opportunistic pathogens in vitro, molecular reagents are urgently needed to develop improved methods to monitor organism load in patients undergoing treatment. The contractor will receive reagents and starting materials from the Project Officer for the purpose of modifying, amplifying, producing, and shipping molecular biology reagents to the NIAID AIDS Research and Reference Reagent Program for distribution to NIAID-supported investigators. It is the objective of this activity to prepare, evaluate, and amplify cDNA and genomic libraries; prepare expression vectors and produce gene products; to prepare, produce, and purify soluble proteins; to prepare and produce polyclonal and monoclonal antibodies; and to assay, evaluate, describe, and ship the resulting reagents to the NIAID and to scientific investigators studying opportunistic infections. The products produced under this contract will be used to support the research activities of the scientific community. The NIAID needs access to significant quantities of the noted materials to support the efforts of the National Cooperative Drug Discovery Groups, the AIDS Clinical Study Groups, the Programs of Excellence in Basic Research on AIDS, and other specific programs initiated by NIAID.