BIOCHEMICAL GENETIC MONITORING IN RODENTS

承包商应：通过电泳法、红细胞裂解物、 和利用七(7)到十二(12)之间的组织匀浆 为每个品系指定的基因座；接受十(10)只近交系或杂交小鼠 每周从两(2)个菌株/杂交种中的每一个；并能够 检测列出的每个基因座所编码的蛋白质或酶 以下为电泳法： 基因座染色体描述 IDH-1 1异柠檬酸脱氢酶 PEP-3 1二肽酶 CAR-2-3红细胞碳酸氢酶 GPD-1 4葡萄糖-6-磷酸脱氢酶 PGM-1 5磷酸葡萄糖变位酶 LDR-1-6乳酸脱氢酶调节剂 GPI-1 7葡萄糖磷酸异构酶 Hb7血红蛋白-β链 ES-18酯酶 Mod-1-9苹果酸酶 ES-3 11酯酶 ES-10 14酯酶 能够监测以下菌株/杂交种。《项目》 如有必要，官员可添加其他品系/杂交种。 A/J NFS/NCR AKR/N RFM/UN BALB/CAN NZB C57BL/6N NZW C57BL/10ScN B10.A

The contractor shall: monitor by electrophoresis, erythrocytelysates, and tissue homogenates utilizing between seven (7) and twelve (12) designated loci for each strain; receive ten (10) inbred or hybrid mice per week from each of two (2) strains/hybrids; and be capable of detecting the proteins or enzymes encoded by each of the loci listed below by means of electrophoresis: Locus Chromosome Description Idh-1 1 isocitrate dehydrogenase Pep-3 1 dipeptidase Car-2 3 RBC carbonic anhydrase Gpd-1 4 glucose-6-phosphate dehydrogenase Pgm-1 5 phosphoglucomutase Ldr-1 6 lactate dehydrogenase regulator Gpi-1 7 glucose phosphate isomerase Hbb 7 hemoglobin-beta chain Es-1 8 esterase Mod-1 9 malic enzyme Es-3 11 esterase Es-10 14 esterase Be capable of monitoring the following strains/hybrids. The Project Officer may add other strains/hybrids if necessary. A/J NFS/NCr AKR/N RFM/UN BALB/cAnN NZB C57BL/6N NZW C57BL/10ScN B10.A