GENETIC MONITORING OF INBREEEDING

本合同的目的是提供遗传监测资源， 确保所有生产设施中近交系的遗传完整性 生产F344/N大鼠和B6 C3 F1杂交小鼠进行毒理学研究， NTP的试验计划以及提供给试验的动物 设施 每种菌株将监测多达15个指定位点，或 通过红细胞裂解物、肾匀浆和 血清蛋白 B6 C3 F1杂交小鼠、杂交小鼠和 来自每个基础殖民地的F344大鼠，生产中心将 定期评估遗传完整性。 遗传 还将通过皮肤移植来评估近交系啮齿动物的完整性。 在 此外，来自B6 C3 F1杂交小鼠的冷冻肾脏接受慢性 试验机构的研究将进行同工酶分析 通过电泳，以确保用于慢性 问题研究 选用Fischer 344/N大鼠和B6 C3 F1杂交小鼠 化学品毒理学和毒理学评价用实验动物种属 DTRT/NTP下的致癌特性。 测试的遗传完整性 动物对于开发可靠和准确的研究数据至关重要， 动物实验。 持续监测生化遗传变异 再衍生以及生产储备和试验动物将确保 NTP动物研究的数据将从遗传同质的 大鼠和小鼠。 试验动物的遗传同质性将是至关重要的， 肿瘤和病变背景发生率的全项目比较 测试实验室之间、化学品之间以及同一个实验室内， 实验室加班

The purpose of this contract is to provide genetic monitoring resource to assure the genetic integrity of inbred stock at all production facilities producing F344/N rats and B6C3F1 hybrid mice to the toxicology research and testing program of the NTP as well as the animals supplied to the testing facilities. Up to 15 designated loci will be monitored for each strain or hybrid by electrophoresis of erythrocyte lysates, kidney homogenates and serum proteins. Parental strains of B6C3F1 hybrid mice, hybrid mice and F344 rats from each of the foundation colonies, and production centers will be evaluated for genetic integrity at periodic intervals. Genetic integrity of inbred rodents will be evaluated by skin grafts also. In addition, frozen kidneys from B6C3F1 hybrid mice received for chronic studies at the testing facilities will be subjected to isoenzyme analysis by electrophoresis to assure genetic integrity of mice used in chronic studies. Fischer 344/N rats and B6C3F1 hybrid mice are the selected experimental animal species for evaluation of chemicals for toxicologic and carcinogenic properties under DTRT/NTP. The genetic integrity of the test animals is essential for developing reliable and accurate research data in animal experiments. Constant monitoring for biochemical genetic variants of rederived as well as production stock and test animals will ensure that data from NTP animal studies will be collected from genetically homogenous rats and mice. Genetic homogeneity of test animals will be essential for program wide comparison of the background incidence of tumors and lesions between testing laboratories, between chemicals and within the same laboratory overtime.