ERA-NET Real-MFM

项目首字母缩略词合作伙伴对Supracon AG的描述，活跃在超导传感器（SQUID）和基于这些传感器的电子系统领域，德国，SME，协调者和系统集成商以及整个系统的卖方和分销商。Supracon参与了不同的研究项目，如THz- videocam (BMBF), THz- two (BMBF), E-SQUID （EC FP7）以及SEMAG （TAB）和OMEGA (ZIM， BMWI): www.supracon.com QMC仪器有限公司成立于1976年，其主要活动是开发，设计和制造定制低温远红外（THz）探测系统，包括无低温单像素和被动成像仪器。产品还包括偏光光谱仪，以及无源元件，如过滤器和偏光器。QMC参与TeraTOP和SpaceKIDs（都是FP7）。www.terahertz.co.uk o德国莱布尼茨光子技术研究所（IPHT）为生命科学和医学开发技术解决方案，重点是生物光子学、光纤和光子检测。在Real-MFM中，IPHT将设计和制造超导单光子探测器，并预组装和测试系统组件。示范实验的计划和执行也将在ipt完成。相关项目：太赫兹摄像机（BMBF），太赫兹two (BMBF), E-SQUID （EC FP7）。www.leibniz-ipht.de o Entropy GmbH是一家低温恒温器制造商，专门从事开尔文和毫开尔文温度范围的闭式循环技术。该公司成立于2010年，总部位于德国慕尼黑。熵设计和制造几种类型的低温恒温器，如温度< 4K的GM和脉管冷却器，温度< 1K的闭式循环焦耳-汤姆逊低温恒温器，绝热消磁冰箱（ADR）和稀释冰箱。http://www.entropy-cryogenics.com/ o Chase Research Cryogenics Ltd，设计，建造和销售定制的亚开尔文冷冻冷却器，主要用于天文学的研究目的，也用于纳米技术和其他领域。主要产品有单级（He-4, He-3）两级（He-7）和三级（He-10）蒸发冷却器。客户包括欧洲的ESTEC和SRON，美国的NASA和NIST，以及欧美许多大学院系。www.chasecryogenics.com项目摘要：在现代医学分析中，荧光显微镜已成为细胞水平生物组织成像不可或缺的工具，从而允许原位研究生命过程。作为一种先进的选择，多重染色技术能够使用特定的荧光团标记细胞中的不同细胞器。多光谱成像可以同时观察多个细胞成分，并研究它们之间的相互作用。以目前的技术，不同波长的信息需要在时间（顺序）或空间（全息）上进行多路复用。顺序方法利用可调谐滤波器或用光谱仪进行像素扫描，而全息技术则从编码孔径计算复杂图像。这两种方法都有缺点和局限性。这里提出的激进方法是使用一个光子探测器，它可以根据时间和能量来区分单个光子。这种技术要求检测性能接近基本物理极限。传统的单光子探测器如雪崩二极管具有较差的能量分辨率，因为这种探测器的带隙与被探测光子的能量相似。相比之下，超导探测器（meV数量级）非常小的能隙使得来自可见光谱的单个光子能够产生多达一千个可供测量的电子。这种探测器最初是为天体物理学研究项目开发的，迄今为止还没有进入工业应用。RealMFM项目旨在展示一种可扩展的超导单光子探测器焦平面阵列，其固有光谱分辨率约为20 nm @ 500 nm波长。我们将使用商业荧光显微镜结合一个适度的成像仪器与64像素。这种独特的组合将用于相关生物样品的成像。这样的探测器阵列承诺了许多独特的功能：•由于时分复用缺点或滤波器/孔径吸收，在没有信号功率损失的情况下获取多光谱图像。•真正的实时成像动态过程（如钙信号瞬态），从而减少问题，如样品光漂白或图像恶化，由于样品/相机运动。•高检测量子效率，可在低激发强度下操作，从而防止样品损坏和快速光漂白。预期结果和开发计划：o我们计划生产一个专门设计用于临床环境的原型研究工具。该技术可以被认为是与标准显微镜一起使用的高端选择。这限制了潜在的市场数量，但这类仪器的高性能构成了技术参考，为新一代诊断技术和仪器铺平了道路。o计划的研发活动会对伙伴的技术和专业知识的进步，以及对中小企业的竞争力产生影响。Supracon将组装计划中的仪器的组件，包括低温平台（Entropy & QMC, Chase）、传感器阵列模块（IPHT）和数据处理（放大和采集）。预计这些结果将促进其他仪器的性能。此外，在Real-MFM项目中开发的多路复用电子设备可以用于焦平面阵列的其他应用，例如在安全方面。技术关键词：生物医学工具，亚开尔文冷却技术，多光谱荧光显微镜，生物医学成像，细胞生物学涉及的工业部门：研究仪器总成本：992.06696欧元公共援助：544.38887欧元（合计）

PUBLISHABLE PROJECT FACT SHEET Project acronym Partners’ descriptions o Supracon AG, active in the field of superconducting sensors (SQUID) and electronic system based on these sensors, Germany, SME, coordinator and system integrator as well as seller and distributor of the entire system. Supracon participates in different research projects like THz-Videocam (BMBF), THz-TWO (BMBF), E-SQUID (EC FP7) as well as SEMAG (TAB) and OMEGA (ZIM, BMWI): www.supracon.com o QMC Instruments Ltd. was founded in 1976 and its main activity is the development, design and manufacture of bespoke cryogenic Far-Infrared (THz) detection systems, including cryogen-free single pixel and passive imaging instruments. Products also include polarizing spectrometers, along with passive components such as filters and polarisers. QMC participates in TeraTOP and SpaceKIDs (both FP7). www.terahertz.co.uk o Leibniz Institute of Photonic Technology, (IPHT), Germany, develops technological solutions for life sciences and medicine with focus on biophotonics, fibre optics and photonic detection. In Real-MFM, IPHT will design and fabricate superconducting single-photon detectors and pre-assemble and test the system components. Planning and execution of the demonstration experiment will also be done at IPHT. Related projects: THz-Videocam (BMBF), THz-TWO (BMBF), E-SQUID (EC FP7). www.leibniz-ipht.de o Entropy GmbH is a cryostat manufacturer specialized in closed-cycle technology for the Kelvin and milliKelvin temperature range. The company was founded in 2010 and is based in Munich, Germany. Entropy designs and manufactures several types of cryostats such as GM and pulse-tube cooler based cryostats for temperatures < 4K, closed-cycle Joule-Thomson cryostats for temperatures < 1K, Adiabatic Demagnetization Refrigerators (ADR) and Dilution refrigerators. http://www.entropy-cryogenics.com/ o Chase Research Cryogenics Ltd, design build and sell custom sub-Kelvin cryo-coolers, mainly for research purposes in Astronomy, but also in nano-technology and other fields. Our main products are single stage (He-4, He-3) two-stage (He-7) and three-stage (He-10) evaporation coolers. Customers include ESTEC and SRON in Europe, NASA and NIST in the USA, as well as many university departments in both Europe and America. www.chasecryogenics.com Project abstract: In modern medical analysis, fluorescence microscopy has become an indispensable tool for imaging of biological tissue at cellular level, thus permitting the study of life processes in situ. As an advanced option, multi-staining techniques are able to tag different organelles in a cell using specific fluorophores. Multispectral imaging makes it possible to observe multiple cell components at the same time, and to study interactions between them.With current techniques, information at different wavelength needs to be multiplexed either in time (sequential) or in space (holographic). The sequential method exploits tuneable filters or pixel scanning with a spectrometer, whereas the holographic technique computes a complex image from, for example, a coded aperture. Both approaches have drawbacks and limitations. The radical approach proposed here is to use a photon detector which discriminates single photons in terms of both time and energy. This technique demands performance in detection which closely approaches fundamental physical limits. Conventional single-photon detectors such as avalanche diodes have poor energy resolution because the band gap of such detectors is similar to that of the energy of the photon to be detected. By contrast, the very small energy gap of superconducting detectors (of order meV) enables a single photon from the visible spectrum to create up to one thousand electrons to be measured.Developed originally for astrophysical research projects, such detectors have not thus far found their way into industrial applications. The RealMFM project, aims to demonstrate a scalable focal-plane array of superconducting single photon detectors with a moderate intrinsic spectral resolution of about 20 nm @ 500 nm wavelength. We will use a commercial fluorescence microscope in conjunction with a modest imaging instrument with 64 pixels. This unique combination will be used to image relevant biological samples. Such a detector array promises a number of unique features:• acquisition of multispectral images without signal power loss due to time-division multiplex disadvantage or filter/aperture absorption.• true real-time imaging of dynamic processes (e.g. calcium signaling transients), thus reducing problems such as sample photobleaching or image deterioration due to sample/camera movement. • high detection quantum efficiency enabling operation at low excitation intensities, thus preventing sample damage and rapid photobleaching.Expected results and exploitation plan: o We plan to produce a prototype research tool specifically designed for use in clinical environments. The technique can be considered as high-end option for use with standard microscopes. This limits the potential market volume, but the high performance of such instruments constitutes a technology reference, paving the way for a new generation of diagnostic techniques and instruments.o The planned R&D activities will have an impact on the advancement of partner’s technologies and special expertise and on the competitiveness of the SMEs. Supracon will assemble the components of the planned instrument, including the cryogenic platform (Entropy & QMC, Chase), the sensor array module (IPHT) and the data handling (amplification and acquisition). It is expected that the results will advance the performance of other instruments. Moreover, the multiplexing electronics to be developed within the Real-MFM project can be exploited for focal-plane arrays for other applications e.g. in security.Technological key words: biomedical tool, sub kelvin cooling technology, multispectral fluorescence microscopy, biomedical imaging, cell biologyIndustrial sectors addressed: instruments for researchTotal cost: 992.066,96 EurosPublic aid granted: 544.388,87 Euros (as an aggregate)