CORE--EVALUATION OF CANDIDATE SIV VACCINES IN RHESUS MONKEY

科学核心将负责体内研究， 疫苗和克隆病毒在猕猴中的应用 SIV：猕猴艾滋病模型。 这个核心将提供进入 SIV：评价新型疫苗策略的猕猴模型 介绍了 所有猴子的临床护理和维护需要 子项目、接种、免疫接种和样本采集将 由该核心的兽医和/或项目负责人执行。 将提供猴组织用于病毒检测;来自感染者的血清 和/或免疫的猴将用于体液免疫分析。 反应和病毒中和抗体。 血清和 细胞将提供细胞介导的免疫的测量。 将提供外周血淋巴细胞用于生成 恒河猴单克隆抗体。 在适当的免疫接种后， 猴将通过静脉内或粘膜用储备液激发 病毒制剂（无细胞或细胞相关）。

The scientific core will be responsible for the in vivo studies with vaccines and cloned viruses in macaques using the well-established SIV:macaque model for AIDS. This core will provide access to the SIV:macaque model for evaluation of the novel vaccine strategies described. The clinical care and maintenance of all monkeys required for the subprojects, inoculations, immunizations, and sample collections will be performed by the veterinarian and/or project leader of this core. Monkey tissues will be provided for virus detection; sera from infected and/or immunized monkeys will be provided for analysis of humoral immune responses and virus neutralizing antibodies, respectively. Sera and cells will provide for measurements of cell mediated immunity. Peripheral blood lymphocytes will be provided for the generation of rhesus monoclonal antibodies. Following appropriate immunization, monkeys will be challenged either intravenously or mucosally with stock virus preparations (cell-free or cell-associated).