THE USE OF CONDUCTING POLYMERS IN BIOMEDICINE

这项建议旨在开展必要的基础研究，以开发 简单的在体葡萄糖传感器。这将包括逐步测试 程序通过生理盐水，人工血清，全血中的血清。我们 都报告说我们的基本战略是合理的，作为一个硼国 官能化的聚噻吩会与葡萄糖反应，导致 薄膜中发生了显著的电化学变化。我们还表明， 简单的乙醇甲醇会在电化学上引起显著的 一种含硼取代聚苯胺的变化。在这个阶段，我们的工作可能 可用于简单的一次检测葡萄糖和甲醇。 然而，需要做的工作是尝试和优化该系统。 我们希望继续进行模板实验，以检测 葡萄糖。我们的初步结果表明，导电聚合物 作为检测系统的一部分，将必须进行交叉链接。这个 研究的第一部分将针对交联实验和 导电膜的稳定性。我们现在已经合成了二硫烯基 并用核磁共振、气相色谱、质谱学和红外光谱对其结构进行了表征。 循环伏安法是目前国内第一个进行交联实验的方法 正在进行中。我们计划用扫描电子来研究这些效应 显微镜、扫描隧道显微镜和X射线光电子 光谱学，以及通常的FT-IR表面效应研究。 一旦这些研究完成，我们将把模板和 确定特异性。 同时，我们将在等渗盐水中测试我们现有的系统 溶液、血清和血浆，看看它们是否可以用于 生物系统。聚噻吩盐溶液的初步研究 解决方案现已完成。聚噻吩膜很快变得稳定 并将提供可重现的交流阻抗谱。简单的酒精 检测将用于乙醇的气相检测。

This proposal is directed at the basic research necessary to develop a simple in vivo glucose sensor. This will include a stepwise testing program going through saline, artificial sera, sera in whole blood. We have reported that our basic strategy is sound, as a borate functionalized polythiophene will react with glucose and cause a significant electrochemical change in the film. We have also shown that the simple alcohol methanol causes an electrochemically significant change in a borate substituted polyaniline. At this stage our work may be useful for simple, one shot detections of glucose and methanol. However, work needs to be done to try and optimize the system. We wish to go ahead with templating experiments for the detection of glucose. Our preliminary results indicate that the conducting polymers used as part of the detection system will have to be cross-linked. The first part of the study will be directed at cross-linking experiments and stability of the conducting films. We have now synthesized dithenyl ethane and characterized the molecule by NMR, G.C. mass spectrometry and cyclic voltammetry Currently the first of the cross-linking experiments are under way. We plan to study these effects using Scanning Electron Microscopy, Scanning Tunneling Microscopy and X-ray photoelectron spectroscopy, along side the usual FT-IR studies on surface effects. Once these studies are done, we will incorporate the template and determine specificity. Concurrently, we will be testing our present systems in isotonic saline solutions, sera, and blood plasma, to see if they can be used in biological systems. The initial studies of polythiophene in saline solution are now complete. The polythiophene films quickly become stable and will give reproducible A.C. impedance spectra. The simple alcohol detection will be used in gas phase detections of ethanol.