GENOME SEQUENCING CENTER

拟建的基因组测序中心将扩大测序能力 CRI正在进行的多重测序项目。 一个额外的焦点 将是新技术和软件的发展， 显著增加了吞吐量。 将有40人参与 两个项目，两个核心。 以下是他们的目标摘要。 项目1：自动多重测序。 总体目标是 序列2.4，4，6.4，11.3和20 Mb连续区域中的五个 连续几年的项目。 增加的吞吐量将是 通过自动化实验室和计算机程序来完成。 的 M. leprae和M.结核病基因组将首先完成。 的 测序靶然后将转移到人类染色体10q24-q26， 小鼠同线区。 总共有24人将参加这次会议。 项目 第5年的预计成本约为每个基地25美分。 项目2：红外探测与自动化。 该项目将重点关注 自动高通量杂交和红外荧光 侦测 红外荧光的使用将显著增加 生产能力，简化质量控制，并通过消除 使用放射性化学品和胶片。 通过集成自动 杂交和检测，所有的手动过程中， 多重测序杂交/检测部分将 淘汰 在五年的时间里，六台机器自动化 杂交和检测将建立，使原始数据的产生， 每年超过500 Mb的速率。 总的来说，7个人将 参与这个项目。 信息学核心。 五年总体目标是发展和 实现能够维持超过1000万像素处理速率的软件 1.5 Mb的原始序列数据和大约100 kb的完成序列 每日 需要解决的四个方面是： 程序，改进序列组装，自动化重叠群编辑，以及 开发工具以促进序列分析。 附加系统 管理和计算任务包括网络和硬件升级， 数据备份、项目管理工具开发和编程 支持机器人自动化。 总的来说，这个核心将涉及8个 个体

The proposed Genome Sequencing Center will expand the sequencing capacity of the ongoing multiplex sequencing project at CRI. An additional focus will be the development of new technology and software in order to significantly increase throughput. Forty individuals will be involved in two projects and two cores. A summary of their goals follows. Project 1: Automated Multiplex Sequencing. The overall goal is to sequence 2.4, 4, 6.4, 11.3 and 20 Mb contiguous regions in the five consecutive years of the project. The increased throughput will be accomplished by automating both laboratory and computer procedures. The M. leprae and M. tuberculosis genomes will be completed first. The sequencing target will then shift to human chromosome 10q24-q26 and its mouse syntenic regions. Overall, 24 individuals will participate in this project. The projected cost in Year 5 is approximately 25 cents per base. Project 2: Infrared Detection and Automation. This project will focus on automated high throughput hybridization and infrared fluorescence detection. The use of infrared fluorescence will significantly increase throughput, simplify quality control, and reduce costs by eliminating the use of radioactive chemicals and films. By integrating automatic hybridization and detection, all manual processes in the hybridization/detection component of multiplex sequencing will be eliminated. Over the course of five years, six machines for automated hybridization and detection will be built, enabling raw data generation at a rate of more than 500 Mb per year. Overall, 7 individuals will participate in this project. Informatics Core. The overall, 5-year objective is to develop and implement software that is capable of sustaining a processing rate of over 1.5 Mb of raw sequence data and approximately 100 kb of finished sequence per day. The four areas to be addressed are: improving base calling routines, improving sequence assembly, automating contig editing, and developing tools to facilitate sequence analysis. Additional system administration and computing tasks include network and hardware upgrades, data backups, development of project management tools, and programming support for robotics automation. Overall, this core will involve 8 individuals.