ANTIBODY ACTION MECHANISM AGAINST CRYPTOCOCCUS NEOFORMAN

说明： 候选人指出，新生隐球菌导致生命 在6-8%的艾滋病患者中，C。 新生杆菌有一个决定毒力的多糖壳。 并且是抗吞噬细胞的，并且胶囊结合抗体是有效的 调理素已被证明能延长小鼠模型的存活时间 在静脉接种真菌之后。然而，真菌进入了 它的宿主通过呼吸树，对它的作用知之甚少 肺部感染中的抗体免疫。这些信息越来越多地 重要的是考虑到正在开发的结合疫苗 抗体的形成。初步数据显示，系统性抗体 预防原发肺部新生芽孢杆菌感染。整体而言 本应用的主题是研究抗体介导的机制 对肺部感染的保护。提出了四个目标。目标1将 抗体改变肺脏病程的疗效测定 感染。目标2将确定血清抗体保护的机制 在肺里。目标3将探讨细胞因子表达对 抗体介导的肺保护作用。目标4将决定 新生假单胞菌肺外传播机制。

DESCRIPTION: The candidate indicates that Cryptococcus neoformans causes life threatening infections in 6-8% of patients with AIDS, that C. neoformans has a polysaccharide capsule which is a determinant of virulence and is antiphagocytic, and that capsule binding antibodies are potent opsonins which have been shown to prolong survival in murine models following intravenous inoculation of the fungus. However, the fungus enters its host through the respiratory tree and little is known about the role of antibody immunity in pulmonary infections. This information is increasingly important given the ongoing development of a conjugate vaccine which elicits antibody formation. Preliminary data indicated that systemic antibody protects against a primary pulmonary C. neoformans infection. The overall theme of this application is to study the mechanism of the antibody mediated protection in pulmonary infections. Four aims are proposed. Aim 1 will determine the efficacy of antibody in modifying the course of pulmonary infection. Aim 2 will determine the mechanism of serum antibody protection in the lung. Aim 3 will explore the contribution of cytokine expression to antibody mediated protection in the lung. Aim 4 will determine the mechanisms of extrapulmonary dissemination of C. neoformans.