INVESTIGATION INTO THE PATHOGENESIS OF CHRONIC SINUSITIS

说明： 候选人：Lisa Wheatley博士于#年在洛约拉大学获得医学博士学位 1986年在芝加哥，并在爱荷华大学接受内科培训。 在那之后，她在印度做了六个月的志愿者，然后 在华盛顿大学接受过敏症和临床免疫学培训 宾夕法尼亚州，在那里她是一个家伙。1994年，她被任命为助理 加州大学内科系教授 弗吉尼亚。她的目标是开发一项新的独立研究计划， 那个机构。 赞助商：托马斯·普拉茨-米尔斯博士是医学教授兼院长， 弗吉尼亚大学过敏和临床免疫学系。 C.环境：研究将在过敏科进行 弗吉尼亚大学健康科学和临床免疫学 中心。 D.培训计划：该项目基于慢性疾病的假设 鼻窦炎是一种炎症性疾病，也可能是免疫性疾病。慢性 鼻窦炎在1986年被估计是最常见的慢性疾病在 在美国，影响了3000多万人。韦奕礼博士建议 通过以下方法探讨IgG4在慢性鼻窦炎中的作用：(1) 血清中常见的细菌抗原的特异性IgG4 鼻窦炎，(2)比较IgG4、IgG1与白蛋白的比值。 鼻腔冲洗和血清，(3)用RT-PCR方法定位鼻窦中的IgG4基因 组织，以及(4)用抗CD40、共培养和细胞因子检测B细胞 生产不含IgE的IgG4。韦奕礼博士还将调查这一角色 慢性鼻窦炎患者Th2细胞因子表达的研究 从炎症鼻窦分离的T细胞的批量培养和克隆 将这些反应与Th1抗原和过敏原的反应进行比较。

DESCRIPTION: a. Candidate: Dr. Lisa Wheatley received her M.D. from Loyola University in Chicago in 1986 and trained in internal medicine at the University of Iowa. Following that, she served as a volunteer in India for six months and then trained in allergy and clinical immunology at the University of Pennsylvania, where she was a fellow. In 1994 she was appointed assistant professor in the Department of Internal Medicine at the University of Virginia. Her goal is to develop a new and independent research program at that institution. b. Sponsor: Dr. Thomas Platts-Mills is Professor of Medicine and Head, Division of Allergy and Clinical Immunology at the University of Virginia. c. Environment: The research will be carried out in the Division of Allergy and Clinical immunology at the University of Virginia Health Sciences Center. d. Training Plan: The project is based on the hypothesis that chronic sinusitis is an inflammatory and perhaps immunological disease. Chronic sinusitis was estimated in 1986 to be the most frequent chronic disease in the U.S., affecting over 30 million individuals. Dr. Wheatley proposes to investigate the role of IgG4 in chronic sinusitis by: (1) detecting, in serum, IgG4 that is specific for bacterial antigens commonly found in inflamed sinuses, (2) comparing the ratios of IgG4 and IgG1 to albumin in nasal washings and serum, (3) using RT-PCR to localize IgG4 mRNA in sinus tissue, and (4) using anti-CD40, coculture, and cytokines to examine B cell production of IgG4 without IgE. Dr. Wheatley will also investigate the role of Th2 responses in chronic sinusitis by measuring cytokine expression by bulk culture and cloned T cells isolated from inflamed sinuses and then comparing these responses to those for Th1 antigen and allergen.