OLFACTORY MECHANISMS OF CORTICOSTEROID ACTION

本研究旨在评估嗅觉系统 粘膜受到肾上腺皮质类固醇激素的调节， 包括盐皮质激素醛固酮，以及糖皮质激素 荷尔蒙肾上腺功能紊乱与 嗅觉异常此外，合成肾上腺皮质类固醇 常用于治疗临床嗅觉障碍 功能障碍尽管如此，关于网站或 皮质类固醇对嗅觉系统的作用机制。 该项目的初步数据表明， 嗅觉粘膜内的皮质类固醇受体。初始目标 该项目将确定的密度和分布 皮质类固醇受体通过使用生化，分子 生物学和免疫组织化学技术。第二次放射自显影 技术将被用来确定密度和分布的 Na，K-ATP酶在嗅粘膜中的表达。这种酶， 在许多其他系统中受肾上腺类固醇调节，存在于高水平 特定分泌和吸收体液的组织中的水平， 电解质实验诱导的循环改变 然后皮质类固醇水平将与Na，K-ATP酶的变化相关 网站.第三，如果肾上腺皮质激素与离子流有关， 嗅觉分泌通过调节Na，K-ATP酶，他们将有 对嗅觉粘液离子组成的影响， 转导原子分光光度法将用于分析 嗅觉粘液离子浓度与 循环皮质类固醇

This research is designed to evaluate the hypothesis that the olfactory mucosa is subject to regulation by adrenal corticosteroid hormones, including the mineralocorticoid aldosterone, as well as glucorticoid hormones. Disorders of adrenal function have been associated with olfactory abnormalities. Furthermore, synthetic adrenal corticosteroids are frequently prescribed for the treatment of clinical olfactory dysfunction. Despite this, little is known regarding the site(s) or mechanism of action of corticosteroids on the olfactory system. Preliminary data for this project has demonstrated the presence of corticosteroid receptors within the olfactory mucosa. initial goals of this project will be to determine the density and distribution of corticosteroid receptors through the use of biochemical, molecular biological, and immunohistochemical techniques. Secondly autoradiographic techniques will be used to determine the density and distribution of the enzyme Na, K-ATPase within the olfactory mucosa. This enzyme, which is regulated by adrenal steroids in many other systems, is present in high levels in tissues specified for secretion and absorption of fluids and electrolytes. Experimentally induced alterations in circulating corticosteroid levels will then be correlated with changes in Na, K-ATPase sites. Third, if adrenal cortical hormones are linked to ion flux and olfactory secretion through the regulation of Na, K-ATPase, they will have an effect on olfactory mucus ion composition and possibly, sensory transduction. Atomic spectrophotometry will be used to analyze changes in olfactory mucus ion concentration associated with alterations in circulating corticosteroids.