POSTTRANSCRIPTIONAL REGULATION OF C-MYC IN C2 MYOBLASTS

C-myc原癌基因编码一种核磷蛋白，它通常 作为转录调节因子发挥作用，被认为是一种 在细胞增殖和分化中发挥重要作用。放松管制 C-myc的表达与肿瘤转化有关。 明确影响c-myc表达的调控机制可能有助于 更好地理解致癌的分子机制。邮寄- 转录基因调控已被证明是一种重要的 C-myc表达的决定因素。拟议的研究旨在 定义决定c-的稳态表达的顺式作用元件 MYC与细胞内c-myc表达下调 并对调控机制进行界定。基于 这些研究的结果，我们将开始表征和确定 介导c-myc调控的跨调控因子。共同监管 将在c-myc和其他短暂的mRNAs之间寻找基序，包括 这些编码其他与细胞增殖有关的因子和 差异化。

The c-myc proto-oncogene encodes a nuclear phosphoprotein which normally functions as a transcription regulatory factor and is thought to play an important role in cell proliferation and differentiation. Deregulated expression of c-myc has been associated with neoplastic transformation. Defining the regulatory mechanisms affecting c-myc expression may help to better understand the molecular mechanisms of carcinogenesis. Post- transcriptional gene regulation has been shown to be an important determinant of c-myc expression. The proposed studies are designed to define the cis-acting elements determining steady-state expression of c- myc and mediating down-regulation of c-myc expression during cellular differentiation and to define the mechanisms of regulation. Based on results of these studies, we will begin to characterize and identify the trans-regulatory factors mediating c-myc regulation. Common regulatory motifs will be sought between c-myc and other short-lived mRNAs, including those encoding other factors involved in cell proliferation and differentiation.