RADIOTHERPY OF HS/TK TRANSDUCED TUMORS BY A VIRAL AGENTS

描述：(改编自调查人员的摘要)总体 这项提案的目标是确定是否将 自杀基因疗法和放射疗法优于单一疗法 形态疗法在人类肿瘤治疗中的应用基于 转导HSV-tk的体外细胞培养研究 人类胶质瘤细胞，将使用三种具体的方法来完成 目标是什么。第一种方法将检验这样一个假设，即肿瘤 其中含有野生型(Wt)的p53功能会对 自杀基因治疗和放射治疗相结合的肿瘤比缺乏 Wt-p53或含有突变的(Mut)p53基因。另外两个重要的 与综合治疗相关的问题需要回答如下：1)是 有足够的放射增敏作用来提高肿瘤的治愈率 价格呢？2)能否开发出合适的输送系统？这 该小组将使用一种具有良好遗传学特征的人类胶质瘤细胞系， U-251细胞，在裸鼠脑内生长。这一点的重大意义 方法将是确定自杀基因的使用是否在 联合放射治疗将提高癌症的治愈率 抗辐射的人类肿瘤。

DESCRIPTION: (adapted from the investigator's abstract) The overall objective of this proposal is to determine whether the combination of suicide gene therapy and radiation therapy is superior to single modality therapy in the treatment of human tumors. Based on the investigators' in vitro cell culture studies with the HSV-tk transduced human glioma cells, three specific approaches will be used to accomplish the objective. The first approach will test the hypothesis that tumors which contain wild type (wt) p53 function will be more sensitive to the combination of suicide gene therapy and radiation than tumors which lack wt-p53 or contain mutated (mut) p53 genes. Two other important questions relevant to the combined therapy to be answered are: 1) Is there sufficient radiosensitization to produce an increased tumor cure rate? and 2) Can an appropriate delivery system be developed? This group will use a genetically well characterized human glioma cell line, U-251 cells, growing in nude rat brains. The major significance of this approach would be to determine whether the use of suicide genes in combination with radiation would improve the cure rates of radioresistant human tumors.