STE2 RECEPTOR CARBOXY TERMINUS AND MATING PRO

在细胞分化时，许多细胞类型表现出两极分化的细胞生长。 形成专门的结构，这对特定的 细胞的功能。我计划研究极化的机制 在酵母的交配细胞中生长，酿酒酵母是 易于服从经典和分子遗传学，是一种有用的 研究极化细胞生长的生物体。暴露时的单倍体酵母细胞 交配信息素阻止细胞生长并在一侧形成突起 在牢房里。来自两个交配伙伴的投射向对方增长 然后引信。面向合作伙伴的预测增长方向是 受信息素梯度水平的影响。Ste2p，a的羧基末端 7-跨膜节段信息素受体，具有大的细胞质尾巴， 对于指导交配投影方向很重要。要理解 受体尾巴如何引导极化生长，我计划精确地 确定负责调节方向的序列。 通过磷酸化对这些序列的调节，以及它们在 交配的其他方面将被调查。最后，我计划确定 与羧基末端取向相互作用的蛋白质 确定它们如何指导细胞生长的序列。这些研究是 预计将提供对外部信号 酵母中的直接细胞形态发生。因为这个过程的许多方面 与在其他系统中观察到的类似，这些研究有望 提供了对真核细胞停滞机制的基本见解 细胞分裂并分化成专门的细胞类型。

Upon cell differentiation, many cell types exhibit polarized cell growth to form specialized structures which are crucial for the particular functions of the cells. I plan to investigate the mechanisms of polarized growth in mating cells of the yeast, Saccharomyces cerevisiae, which is readily amenable to classical and molecular genetics, and is a useful organism to study polarized cell growth. Haploid yeast cells when exposed to mating pheromones arrest cell growth and form a projection on one edge of the cell. Projection from two mating partners grow toward one another and fuse. The orientation of projection growth toward the partner is directed by pheromone gradient levels. The carboxy terminus of Ste2p, a 7-transmembrane segment pheromone receptor with a large cytoplasmic tail, is important for directing mating projection orientation. To understand how the receptor tail directs polarized growth, I plan to precisely determine the sequences which are responsible for mediating orientation. The regulation of these sequences by phosphorylation, and their role in other aspects of mating will be investigated. Finally, I plan to identify the proteins which interact with the carboxy terminal orientation sequences to determine how they direct cells growth. These studies are expected to provide insights into the mechanism by which external signals direct cell morphogenesis in yeast. Because many aspects of this process resemble those observed in other systems, these studies are expected to provide basic insights into mechanisms by which eucaryotes cells arrest cell division and differentiate into specialized cell types.