REGULATION OF THE DEVRS OPERON IN MYXOCOCCUS XANTHUS

由于其在黄色粘球菌子实体中的关键作用， 形成及其作为遗传开关的潜在作用，devRS操纵子 将进一步分析，强调负自动调节。的 将首先通过在以下基因中进行缺失来鉴定自身调节蛋白： 确定哪些基因会影响 的RNA。然后，将确定该法规是否处于 RNA的稳定性或在转录水平上的抑制。的RNA 将通过测定RNA在细胞中的半衰期来检查稳定性。 野生型和突变株。如果在这些菌株中没有差异， 然后检查转录的抑制。启动子区 将首先被测序，转录起始位点将被 使用S1作图和引物延伸测定。然后一个突变 将进行启动子区的分析以确定位点 需要镇压。在此之后，自动调节蛋白将 使用目前的融合蛋白技术纯化，并与 将分析RNA或DNA。

Because of its critical role in Myxococcus xanthus fruiting body formation and its potential role as a genetic switch, the devRS operon will be further analyzed, emphasizing the negative autoregulation. The autoregulatory protein will first be identified by making deletions in the genes identified and determining which one affects the accumulation of RNA. Then, it will be determined if the regulation is at the level of RNA stability or at the level of repression of transcription. The RNA stability will be examined by determining the half-life of the RNA in the wild type and mutant strains. If it is not different in these strains, then repression of transcription will be examined. The promoter region will first be sequenced and the transcriptional start site will be determined using S1 mapping and primer extension. Then a mutational analysis of the promoter region will be performed to determine sites required for repression. After this, the autoregulatory protein will be purified using current fusion protein technology and interactions with the RNA or DNA will be analyzed.