HLH GENE FUNCTION IN NEURONAL CELL DETERMINATION

描述：这项建议旨在了解 一组编码转录因子家族的基因的功能 它们在神经元发育中起着核心作用。BHLH蛋白 由正负向调节器组成的一个大家族 抄写。积极的监管机构分为两类：一类是单一的 常见的正向调节器被称为无女儿，因其 在性别决定中的作用，形成了一个与 来自无毛刺毛复合体的蛋白质。这些异源二聚体结合到 称为E盒的特定调控序列。消极的监管者倒下 分为两类：EMC蛋白是一种不含DNA的HLH蛋白 结合结构域。它通过抑制形成来抑制转录。 达海特/Scute杂二聚体。第二类，包括产品 分裂基因的增强子和毛发结合到一组不同的 与E框相关但不同的序列。一套优雅的 考迪博士在《基因与发育》中发表的令人信服的实验 证明毛茸茸的行为可以抑制无毛类动物的活动。 通过直接与DNA结合的靶向启动子。

DESCRIPTION: This proposal is directed towards understanding the function of a set of genes encoding a family of transcription factors which play a central role in neuronal development. The bHLH proteins comprise a large family of both positive and negative regulators of transcription. The positive regulators fall into two classes: a single common positive regulator is called daughterless, so named for its function in sex determination, forms a heterodimeric complex with proteins from the achaete scute complex. These heterodimers bind to specific regulatory sequences called E box. The negative regulators fall into two classes: The emc protein is an HLH protein devoid of a DNA binding domain. It represses transcription by inhibiting the formation of da achaete/scute heterodimers. The second class including the products of the enhancer of split locus and hairy bind to a distinct set of sequences related to but different from the E box. An elegant set of experiments published by Dr. Caudy in Genes and Development convincingly demonstrate that hairy acts to repress the activity of an achaete/scute target promoter by direct binding to DNA.