LENTIVIRUS MUTATION AND REVERSE TRANSCRIPTASE FIDELITY

描述：这些研究旨在确定是否改变 慢病毒逆转录酶保真度通过促进 抗药性和宿主范围病毒变异的出现。 研究 将采用模型慢病毒（猫免疫缺陷病毒- FIV）， 单循环病毒复制（dsDNA-ssRNA- dsRNA），以及敏感的 细胞培养空斑试验，以量化耐药性和宿主范围 变体。 具体来说，研究人员的目标是确定FIV的发生率， 突变对耐药和宿主范围变异的影响， 的RT保真度的突变率，并确定FIV突变体与mutator和 抗突变体表型。 最终，有趣的FIV变异与改变 复制保真度可以用来感染动物，以确定是否 RT保真度的改变导致毒力、 耐药性或抗原变异;然而，体内研究并不 当前应用程序的一部分。

DESCRIPTION: These studies seek to determine if mutations that alter lentivirus reverse transcriptase fidelity alter pathogenesis by promoting the emergence of drug-resistant and host range viral variants. The studies will employ a model lentivirus (feline immunodeficiency virus - FIV), a single cycle of virus replication (dsDNA -- ssRNA -- dsRNA), and a sensitive cell culture plaque assay to quantify drug-resistant and host range variants. Specifically, the investigators aim to determine the rate of FIV mutation to drug-resistant and host range variants, determine the influence of RT fidelity on mutation rate, and identify FIV mutants with mutator and antimutator phenotypes. Ultimately, interesting FIV variants with altered replication fidelity might be used to infect animals to determine whether alterations in RT fidelity lead to alterations in virulence, patterns of drug resistance or antigenic variation; however, in vivo studies are not part of the current application.