CELL ADHESION MOLECULES IN CARDIOGENESIS

描述（摘自申请人摘要）：聚唾液酸化的作用 NCAM在心脏传导系统的正常发育中会 在鸡胚模型中进行研究。神经细胞粘附 分子NCAM及其聚唾液酸部分是细胞-细胞免疫调节剂， 在发育中的心脏中发现的相互作用。上下调节 在神经和骨骼肌发育过程中， 对于创造平衡，导致正常的形态发生至关重要 模式.在之前的研究中，申请人已经证明NCAM 和PSA在发育中的心脏组织中的表达受到调节。 她还表明，扰动NCAM或PSA改变功能， 连接心肌细胞和富含PSA的NCAM的缝隙连接， 在心室传导组织中更常见这些发现 导致调查人员提出，NCAM和PSA的行为，因为他们 对神经元过程的作用，以允许可塑性的相互作用， 心肌细胞，特别是传导组织的细胞， 心脏发生本研究的目的是：1）完善这一假设 通过确定PSA在尚未确定的区域和阶段中的分布， 详细研究;和2）通过酶促反应来测试他们的假设 从卵内胚胎心脏中取出PSA， 形态学、生物化学和电生理学的后果 传导系统。

DESCRIPTION (taken from applicants abstract): The role of polysialated NCAM in the normal development of the cardiac conduction system will be investigated in the chicken embryo model. The neural cell adhesion molecule NCAM and its polysialic acid moiety are modulators of cell-cell interactions found in the developing heart. Up and down regulation of these components during neural and skeletal muscle development is critical for creating the balance that results in normal morphogenetic patterns. In previous studies, the applicant has demonstrated that NCAM and PSA are regulated in their expression in developing heart tissue. She has also shown that perturbation of NCAM or PSA alters the function of gap junctions which couple myocardial cells and PSA-rich NCAM is found preferentially on ventricular conduction tissue. These findings has led the investigator to propose that NCAM and PSA are acting as they do on neuronal processes to allow plasticity in interactions of myocardial cells, particularly those of conduction tissue during cardiogenesis. The aims of this study are: 1) to refine this hypothesis by determining the PSA distribution in regions and stages not yet studied in detail; and 2) to test their hypothesis by enzymatically removing PSA from the embryonic heart in ovo and assessing the consequences to the morphology, biochemistry and electrophysiology of the conduction system.