MOLECULAR CLONING OF HUMAN PLATELET GLYCOPROTEIN IB

描述：这些研究的长期目标是了解 血管性血友病受体GP Ib在分子水平的结构和功能 水平 该受体由4个亚单位组成，GP Ib α，GP Ib β，GP Ib β，GP Ib α，GP Ib β，GP Ib β和GP Ib β。 剪切力影响GP Ib和von之间的相互作用 而Willebrand因子的第一个具体目的是确定剪切是否 影响GP Ib、血管性血友病因子或两者。 在这个特定的实验中， aim还将表征剪切诱导的 可溶性和固定化的血管性血友病因子与GP Ib的结合。的GP Ib-von Willebrand因子相互作用导致细胞内变化， 第二个具体目标是确定GP的细胞内结构域如何 Ib复合物参与了这种跨膜信号传导。 GP Ib的作用 β磷酸化，蛋白质14-3-3，和肌动蛋白结合蛋白在这一过程中， 信号将被评估。

DESCRIPTION: The long term goal of these studies is to understand the structure and function of the von Willebrand receptor GP Ib at a molecular level. This receptor consists of 4 subunits, GP Ib alpha, GP Ib beta, GP IX, and GP V. Shear forces affect the interaction between GP Ib and von Willebrand factor and the first specific aim is to determine whether shear affects GP Ib, von Willebrand factor, or both. Experiments in this specific aim will also characterize the binding domains involved in the shear-induced binding of soluble and immobilized von Willebrand factor to GP Ib. The GP Ib-von Willebrand factor interaction leads to intracellular changes and the second specific aim is to determine how the intracellular domains of the GP Ib complex participate in this transmembrane signaling. The role of GP Ib beta phosphorylation, protein 14-3-3, and actin-binding protein in this signaling will be assessed.