NOVEL P36 MBP KINASE IN CERAMIDE-INDUCED APOPTOSIS

描述（改编自研究者摘要）：P.I.此前曾 证明了36 kDa髓鞘碱性蛋白激酶（MBP激酶）是 神经酰胺诱导HL-60早幼粒细胞白血病细胞凋亡过程中的激活 细胞 这种激酶在细胞亚群中被激活， 启动凋亡。 纯化和微测序显示， 激酶来源于先前鉴定的哺乳动物不育20 (MST)激酶，其两种同种型被克隆并通过 私家侦探 私家侦探现在计划：1）表征细胞调节， MST激酶对应激/凋亡信号的响应; 2）表征MST激酶对应激/凋亡信号的响应。 使用诱导表达系统研究这些激酶的生理作用 和基因工程突变体，以及3）表征信号传导 导致MST激酶激活的信号通路。

DESCRIPTION (adapted from investigator's abstract): The P.I. has previously demonstrated that a 36kDa Myelin Basic Protein Kinase (MBP Kinase) is activated during ceramide-induced apoptosis in HL60 promyelocytic leukemia cells. This kinase is activated in the subset of cells that have actually initiated apoptosis. Purification and microsequencing reveal that the kinase is derived from a previously identified Mammalian Sterile Twenty (MST) Kinase, two isoforms of which were cloned and characterized by the P.I.. The P.I. now plans to: 1) characterize the cellular regulation of MST kinases in response to stress/apoptotic signals; 2) characterize the physiological roles of these kinases using an inducible expression system and genetically engineered mutants and 3) characterize the signalling pathway that leads to activation of the MST kinases.