NITRIC OXIDE ACTION IN PRIMATE MYOMETRIUM

描述（改编自研究者摘要）：在这两次 修订的新R29应用程序，迈克尔E.布拉德利博士申请35万美元 研究“一氧化氮的分子基础”的直接费用超过5年 在灵长类子宫肌层中的作用。“这个项目的长期目标是 了解子宫收缩开始的信号 与劳动有关。 本提案的具体目的是确定， 人类或猴子的子宫组织产生一氧化氮， 一氧化氮可使人或猴子宫肌细胞松弛。 该提案 分为两个具体目标。 在具体目标1中，研究人员将 确定外源性NO在非妊娠、近足月妊娠、 以及子宫组织和细胞的分娩。 在这一具体目标中， 研究者将确定NO对子宫肌层组织张力的影响 并测定NO对细胞内钙、cAMP和 cGMP。 在具体目标2中，研究者将描述、定位和 探讨一氧化氮合酶亚型在灵长类动物子宫中的调节作用。 在这一特定目标中探索的技术包括Western印迹分析 完整组织和培养细胞的NOS mRNA的测定 使用RT-PCR表达。

DESCRIPTION (Adapted from the Investigator's Abstract): In this twice revised new R29 application, Michael E. Bradley, Ph.D. requests $350,000 in direct costs over 5 years to study the "molecular basis of nitric oxide action in primate myometrium." The long-term goal of this project is to understand the signals responsible for commencement of uterine contractions associated with labor. The specific aim of this proposal is to determine if the tissues of the human or monkey uterus produce nitric oxide and whether nitric oxide can relax human or monkey uterine myocytes. The proposal is divided into 2 specific aims. In specific aim 1 the investigator will determine the effects of exogenous NO in non-pregnant, near term pregnant, and laboring uterine tissues and cells. In this specific aim the investigator will determine the effects of NO on myometrial tissue tension and determine the effects on NO on the concentration of calcium, cAMP and cGMP. In specific aim 2 the investigator will characterize, localize and investigate the regulation of NOS isoforms in the primate uterus. Techniques to be explored in this specific aim include Western blot analysis of intact tissues and cultured cells and determination of NOS mRNA expression using RT-PCR.