SCREENING OF THERMOPHILES FOR ANTIFUNGAL ACTIVITY

描述：鉴定新的生物活性化合物变得越来越重要 难度越来越大。已经筛选出许多潜在的来源。 无数次，减少了找到额外的、有用的 材料。避免这种绊脚石的一种方法是识别小说 微生物。其中一个更有成效的地方是找到未经考验的地方 生物体是搜索在很大程度上未被探索但已知的栖息地 包括活跃的微生物群落。此应用程序建议 从热水微生物席中发现的微生物中筛选出提取物 还有抗真菌活性的烟雾剂。 此第一阶段应用程序的两个目标是：1.集成 测试样品抗真菌活性的一组筛子。这些遗嘱 包括全细胞分析、体外酶筛选和os-1细胞壁 筛选；以及2.测试嗜热菌的抗真菌活性。 这项工作将鉴定产生抗真菌抗生素的微生物。 来自之前未经过筛选的环境。很可能这些都是 化合物将是新的，并导致新的抗真菌治疗药物。

DESCRIPTION: The identification of novel bioactive compounds is becoming increasingly more difficult. Many potential sources have been screened numerous times, decreasing the possibility of finding additional, useful materials. One way to avoid this stumbling block is to identify novel microorganisms. One of the more fruitful places to find untested organisms is to search habitats that are largely unexplored but are known to include active microbial communities. This application proposes to screen extracts from organisms found in microbial mats in thermal waters and from fumaroles for antifungal activity. The two objectives in this Phase I application are: 1. To integrate a battery of screens to test samples for antifungal activity. These will include whole cell assays, in vitro enzyme screens and os-1 cell-wall screen; and 2. To test thermophilic organisms for antifungal activity. This work will identify organisms that produce antifungal antibiotics from previously unscreened environments. It is likely that these compounds will be novel and lead to new antifungal therapeutics.