Targeted genome editing using engineered CRISPR-Cas endonucleases

本项目旨在研究靶向性和细胞特异性内切酶的生成。CRISPR-Cas内切酶由于其基于短向导RNA的编程简单和高切割效率，已经彻底改变了基因组工程领域。该项目将结合基因组工程和抗体治疗两种技术，产生新的抗体靶向内切酶，以高效率和特异性修饰细胞基因组。该项目将为酒窖功能提供新的、有趣的见解，在基础研究和生物技术方面具有广泛的应用。

This project aims to study the generation of targeted and cell-specific endonucleases. CRISPR-Cas endonucleases have revolutionised the field of genome engineering due to programming simplicity based on a short guide RNA and high cleavage efficiency. This project will combine the use of two technologies in genome engineering and antibody therapeutics to generate new antibody-targeted endonucleases that modify cellular genomes with high efficacy and specificity. This project will provide new and intriguing insights into cellar function, with broad applications in basic research and biotechnology.