ANTIGENIC ANALYSIS OF DENGUE VIRUS ENVELOPE PROTEIN SUBUNIT VACCINES

登革热(登革热)病毒继续在世界各地造成重大流行病 世界上的热带和亚热带地区。在目前的时间里 没有登革热病毒疫苗可用。巢状病毒粒子包膜(E) 蛋白质刺激保护性病毒中和抗体，是一种 理想的疫苗候选者。已经进行了生物合成的工作 在两种表达系统中产生真实的登革热病毒E蛋白。辛德比斯 (SIN)病毒复制和重组痘苗病毒。修改 登革热病毒克隆基因插入含有羧基末端区域 登革热病毒衣壳蛋白的表达和分泌 PRM/E蛋白在两种系统中的表达。PRM/E蛋白水平升高 分泌到培养液中时至少有15个氨基酸的羧基 衣壳蛋白的一部分和切割部位的碱性氨基酸 都包括在内。分泌型PRM/E颗粒可与可溶性PRM/E颗粒分离 蛋白质通过牙本质分离，可能代表着缓慢的沉着 血凝素(SHA)。目前正在进行研究，以增强 这些抗原的产生和启动的单克隆抗体分析 它们的抗原性结构，刺激中和抗体的能力 并提供免受登革热病毒感染的保护。

Dengue (DEN) viruses continue to cause major epidemics throughout the tropical and subtropical regions of the world. At the present time there are no dengue virus vaccines available. The DEN virion envelope (E) protein stimulates protective virus neutralizing antibodies and is an ideal vaccine candidate. Work has been undertaken to biosynthetically produce authentic DEN virus E protein in two expression systems. Sindbis (SIN) virus replicons and recombinant vaccina viruses. Modification of the DEN virus cloned gene insert to include the carboxyl terminal region of the DEN virus capsid protein facilitated expression and secretion of prM/E protein by both systems. Enhanced levels of prM/E protein were secreted into the medium when at least 15 amino acids of the carboxyl portion of the capsid protein and basic amino acids of the cleavage site were included. Secreted prM/E particles could be separated from soluble proteins by dentrifugation and probably represent the slowly sedementing hemagglutinin (SHA). Studies are currently underway to enhance production of these antigens and initiate monclonal antibody analysis of their antigenic structure, ability to stimulate neutralizing antibodies and provide protection from DEN virus infection.