STUDIES OF HUMAN T-HELPER CELL TYPE 1 AND TYPE 2 CYTOKINE RESPONSES

T辅助细胞已被划分为不同的子集 它们分泌的细胞因子。THO细胞分泌IL2、IL4和IFNG；TH1细胞 分泌IL2和IFNG；TH2细胞分泌IL4。易感性 某些传染病已被证明依赖于 诱导出优势类型的TH细胞。有一些证据表明 HIV-1感染的进展与偏向TH2有关 细胞。因此，如果针对HIV-1感染的疫苗 通过招募Th1而不是Th2细胞来诱导抗HIV免疫。胡麻B. 流产已被证明能在小鼠体内诱导TH1细胞。因为流产杆菌可以 也以一种T不依赖的方式刺激人类B细胞 研究它作为HIV-1蛋白和多肽的可能载体 疫苗。因此，有兴趣确定B。 流产对人类T细胞的影响。静息状态下的小T细胞 表达IFNG和IL-2，但不表达IL-4。 这些结果表明，流产杆菌可以刺激TH1反应。 人类T细胞，因此作为人的载体可能是有益的 感染了HIV-1病毒。由于TH1响应在以下情况下受到青睐 IL-12，单核细胞产物，我们研究了流产杆菌是否能诱导 人单核细胞释放IL-12。MRNA的聚合酶链式反应显示，流产杆菌 诱导单核细胞产生IL-12，为B细胞提供了第二条途径。 以诱导类似TH1的反应。细胞因子可能在 接受免疫球蛋白IV治疗后出现的不良反应。对此进行了研究 使用人类T细胞和单核细胞。免疫球蛋白Ig IV可诱导TnFa， 人单核细胞产生IL-1β和IL-6。这些结果可能解释了 患者经常出现的发烧、头痛和腰背痛 收到这款产品。

T helper cells have been divided into different subsets based on the cytokines they secrete. THO cells secrete IL2, IL4 and IFNg; TH1 cells secrete IL2 and IFNg; and TH2 cells secrete IL4. Susceptibility to certain infectious diseases has been shown to be dependent on the predominant type of TH cell induced. There is some evidence that progression of HIV-1 infection is associated with a bias towards TH2 cells. Thus, vaccines for HIV-1 infection may be advantageous if they induce anti-HIV immunity by recruiting TH1 rather than TH2 cells. B. abortus has been shown to induce TH1 cells in mice. Since B. abortus can also stimulate human B cells in a T-independent manner we have been studying it as a possible carrier for HIV-1 proteins and peptides as a vaccine. It was therefore of interest to determine what effect B. abortus would have on human T cells. Small resting human T cells were shown to express IFNg and IL2, but not IL4 in terms of protein and mRNA. These results indicate that B. abortus can stimulate TH1 responses from human T cells, and as such may be beneficial as a carrier for persons infected with HIV-1. Since TH1 responses are favored in the presence of IL-12, a monocyte product, we studied whether B. abortus could induce IL-12 release from human monocytes. PCR of mRNA revealed that B. abortus does elicit IL-12 from monocytes, providing a second pathway for B. abortus to induce a TH1-like response. Cytokines may play a role in the adverse effects seen following treatment with IGIV. This was studied using human T cells and monocytes. IGIV was shown to induce TNFa, IL-1beta and IL-6 from human monocytes. These results may explain the fever, headache and low back pain frequently experienced by patients receiving this product.