IN VITRO POTENCY ASSAY DEVELOPMENT FOR TETANUS ANTITOXIN

本研究旨在开发一种体外效价测定方法， 正式放行试验的豚鼠死亡试验部分 吸附类毒素疫苗中的破伤风成分。 以下已发表 文学，与作者取得了联系，时间花在了他的作品上。 实验室学习酶联免疫吸附剂的力学 豚鼠破伤风毒素/类毒素IgG型抗体的试验（ELISA）。 采用常规方法检测的动物合并血清的等分试样 用于ELISA评价。 相关的反应 通过ELISA测量抗破伤风抗体的存在;然而， 测定的定量，以中和的特定单位表示 活性、mg抗体或与标准品的相对效价仍低于 评价 一旦该方法最终确定， 计划好了

This study is designed to develop an in vitro potency assay to replace the guinea pig death test portion of the official release test for the tetanus component in adsorbed toxoid vaccines. Following published literature, contact was made with the author, and time was spent in his laboratory to learn the mechanics of an Enzyme Linked Immuno Sorbent Assay (ELISA) for guinea pig IgG type antibodies to Tetanus toxin/toxoid. Aliquots of pooled sera from animals tested using the conventional method were set aside for evaluation by ELISA. Responses correlating to the presence of anti-tetanus antibody were measured by ELISA; however, quantitation of the assay, in terms of specific units of neutralizing activity, mg antibody or relative potency to a standard is still under evaluation. Once the method is finalized, collaborative studies are planned.