Investigation of inhibition of current supplies of Immun

免疫电流供应抑制的研究

• 批准号: 6682271
• 负责人: M C ANDERSON
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6682271
• 关键词: Vero cells; antiviral agents; bioassay; diphtheria toxin; drug screening /evaluation; immunoglobulins; passive immunization; technology /technique development; vaccinia virus; viral vaccines

Summary: LST is currently evaluating several Vero Cell Assays as replacements for current animal tests; one assay involves determining the neutralizing activity of Immune Globulins(IG) on Diphtheria Toxin. With some assay modifications, lots of IG were examined for their ability to inhibit infection of Vero Cells by the current CBER Reference Smallpox vaccine, which has been shown to be active in a traditional plaque assay with several cell types. While still under development, preliminary results have indicated that current lots of IG have varying ability to protect Vero Cells in this assay.

摘要：LST目前正在评估几种Vero细胞测定方法，以替代目前的动物试验；一种检测方法是测定免疫球蛋白（IG）对白喉毒素的中和活性。经过一些试验修改，许多IG被CBER参考天花疫苗检测了它们抑制Vero细胞感染的能力，该疫苗已被证明在几种细胞类型的传统斑块试验中具有活性。虽然仍在开发中，但初步结果表明，目前许多IG在该试验中具有不同的保护Vero细胞的能力。