DIAGNOSTIC ELECTRON MICROSCOPY AND PEDIATRIC PATHOLOGY

该方案的目的是检测其毒性和免疫学特性。 巨噬细胞集落刺激因子和肿瘤坏死因子在PTS中的调节作用。癌症晚期。这个 GM-CSF联合肿瘤坏死因子促进树突状细胞成熟 CD34+骨髓祖细胞体外培养。肿瘤坏死因子有双重作用 在树突状细胞成熟中的作用，它上调了 粒-巨噬细胞集落刺激因子受体在骨髓祖细胞上的表达及预防 它们会成熟为粒细胞。树突状细胞是最有效的 免疫系统的抗原提呈细胞。它们作为引发剂发挥作用 在器官移植排斥反应中，主要组织相容性限制T细胞 在反应和抗体的形成依赖于T细胞。 树突状细胞在许多组织和组织中有少量存在。 外周血液。本研究将检测肿瘤坏死因子和粒细胞集落刺激因子的活性。 刺激树突状细胞的成熟和功能。表皮层 用S100染色和外周血计数朗格汉斯细胞 用树突状细胞集落形成单位分析单个核树突状细胞前体细胞 纤维素。治疗后表皮中的S100+细胞增加了一倍(2.55 治疗前至治疗后5.98，p=0.029)。树突状菌落 3例患者治疗后外周血中形成单位增多 结直肠癌患者，但在PTS中没有增加。使用 黑色素瘤。该协议的主要目标是确定是否 GM-CSF联合肿瘤坏死因子诱导树突状细胞成熟 增加血液、骨髓和皮肤中它们的数量，并确定 如果这种增加会导致肿瘤的退化。

The purpose of this protocol is to examine the toxicity and immuno- modulatory activity of GM-CSF and TNF in pts. with advanced cancer. The combination of GM-CSF and TNF stimulates the maturation of dendritic cells from CD34+ bone marrow progenitor cells in vitro. TNF has a two-fold function in the maturation of dendritic cells, it up-regulates the expression of the GM-CSF receptor on bone marrow progenitors and prevents their maturation into granulocytes. Dendritic cells are the most potent antigen presenting cells of the immune system. They function as initiators in organ transplant rejection, major histocompatibility restricted T cell responses and in the formation of antibodies dependent on T cells. Dendritic cells are found in small numbers in many tissues and in peripheral blood. This study will examine the activity of TNF and GM-CSF to stimulate the maturation and function of dendritic cells. Epidermal Langerhans cells were quantitated by S100 staining and peripheral blood mononuclear dendritic cell precursors by dendritic CFU assay in methy- cellulose. S100+ cells in the epidermis doubled after treatment (2.55 before treatment to 5.98 after treatment, p=0.029). Dendritic colony forming units in peripheral blood were increased after treatment in 3 patients with colorectal cancer but were not increased in pts. with melanoma. The major goal of this protocol is to determine whether the combination of GM-CSF and TNF causes maturation of dendritic cells and increases their number in the blood, bone marrow and skin and to determine if this increase produces tumor regressions.