CORE--TRANSGENIC/VIRAL VECTORS

RCAS/RCAN逆转录病毒载体被广泛用作研究 在鸟类系统中的发育和肿瘤发生。 建立 表达这些载体的特异性受体的转基因小鼠 大大提高了系统的灵活性。 我们已经开发 转基因小鼠品系，其在一种受体的控制下表达受体。 组织特异性启动子;这使得有可能将载体引导至 特定的细胞或组织。 这个系统可以用于血统 分析;它将简化致癌基因的影响的研究， 体内形态发生;此外， 这意味着这可以是一个有效的模型系统来评估 基因治疗策略 我们特别感兴趣的是 应用该系统研究骨骼肌老化的影响; 然而，我们要指出，使用 RCAS/RCAN载体和A亚群受体小鼠的适当品系均 来研究和测试其他组织的治疗策略。

The RCAS/RCAN retroviral vectors are in wide use as tools for studying development and oncogenesis in the avian system. The creation of transgenic mice that express a specific receptor for these vectors has significantly increased the flexibility of this system. We have developed lines of transgenic mice that express the receptor under the control of a tissue-specific promoter; this makes it possible to direct the vector to particular cells or tissues. This system can be used for lineage analysis; it will simplify studies of the effects of oncogenes and morphogenesis in vivo; in addition, the ability to make defined mutations in the mouse means that this can be an efficient model system to evaluate gene therapy strategies. We are particularly interested in the applications of this system to study the effects of aging skeletal muscle; however, we would point out that it should be relatively simple to use the RCAS/RCAN vectors and appropriate strains of subgroup A receptor mice both to study and test therapeutic strategies in a variety of other tissues.