GLUCOCORTICOID CONTROL OF LEYDIG CELL DEATH AND MITOSIS

描述 这个项目的长期目标是确定机制(S) 因此GCs抑制男性的生殖功能。GCS之前已经 显示出直接在睾丸水平上的作用，并抑制 编码睾酮生物合成酶的基因的表达。GCS 也被证明可以控制细胞的增殖和 其他细胞类型中的细胞凋亡。第一和第二个具体目标 建议是检查在体内或在体内注射GCs的效果 体外对间质细胞有丝分裂和凋亡的影响。的第三个具体目标 建议测定间质细胞c-jun的表达水平 GCs在体内和体外的治疗。在所有特定的目标中，细胞将 从跨越关键发育阶段的3个不同年龄的大鼠身上制备 间质细胞的成熟，并将用GC水平来处理 要么反映正常环境，要么反映 压力状态。

DESCRIPTION The long-term objectives of this project are to determine the mechanism(s) whereby GCs suppress male reproductive function. GCs have been previously show to have actions directly at the level of the testis, and to inhibit the expression of genes which encode testosterone biosynthetic enzymes. GCs have also been shown to control processes of cell proliferation and apoptosis in other cell types. The first and second specific aims of the proposal are to examine the effects of GCs administered either in vivo or in vitro on Leydig cell mitosis and apoptosis. The third specific aim of the proposal is to determine the level of c-jun expression in Leydig cells after treatment with GCs in vivo and in vitro. In all specific aims, cells will be prepared from rats of 3 different ages which span key development stages of the Leydig cell maturation, and will be treated with levels of GC which reflect either the normal milieu or that which would be expected under conditions of stress.