South Australian High Throughput Screening Facility

高通量筛选设施：该项目旨在建立高通量筛选设施(包括共聚焦细胞成像仪和表面等离子共振设施)，使研究人员能够获得设备，使培养细胞能够用大型化学物质或核酸(过度表达、RNA干扰、微RNA、长非编码RNA)库进行筛选。细胞反应可以通过生化分析或详细成像进行监测。这些筛选将使解剖生物途径和发现调节基本生物过程的基因成为可能。模型将包括微生物、植物和哺乳动物细胞。该设施还将研究分子间的相互作用。基于片段的铅发现屏幕将产生用于研究蛋白质和膜生物学的新型试剂。

High throughput screening facility: The project aims to establish a high throughput screening facility (including a confocal cell imager and surface plasmon resonance facility) to provide researchers with access to equipment that will enable cultured cells to be screened with large libraries of chemicals or nucleic acids (over-expression, RNA interference, microRNAs, long non-coding RNAs). Cellular responses can be monitored by biochemical assays or detailed imaging. These screens will enable dissection of biological pathways and discovery of genes that regulate fundamental biological processes. Models will include microbial, plant and mammalian cells. The facility will also study inter-molecular interactions. Fragment-based lead discovery screens will generate novel reagents for studying protein and membrane biology.