BREATH TEST FOR HEART TRANSPLANT REJECTION

描述：（改编自申请人摘要）：心脏移植 排斥反应伴随着氧自由基活性的增加。 这些氧自由基通过脂质过氧化作用降解膜， 释放挥发性有机化合物（VOC），这些化合物被排出体外， 呼吸因此，呼吸VOC分析可以检测排斥的标记。 在28例心脏移植患者中分析了呼吸VOC， 肌内膜活检10种挥发性挥发性有机物（包括戊烷） 在有组织学证据的患者中， 排斥反应一个判别分析模型，采用这些挥发性有机化合物识别 排斥反应的敏感性为83.3%，特异性为100%。研究者 假设呼吸测试挥发性有机化合物可以提供一个有用的新的早期 心脏移植排斥反应的诊断测试。实验计划 将：1）在200例心脏移植患者中进行呼吸VOC分析 接受肌内膜活检的患者，2）识别显著VOC 拒绝的标记，并使用判别式将这些标记联合收割机组合在模型中 分析和3）确定敏感性，特异性和预测 呼吸测试的价值。研究的长期目标是 确定呼吸测试是否可以检测心脏移植排斥反应， 减少常规肌内膜活检的需要。 拟定商业应用： 潜在的商业应用不可用。

DESCRIPTION: (Adapted from applicant's abstract): Heart transplant rejection is accompanied by increased oxygen free radical activity. These oxygen free radicals degrade membranes by lipid peroxidation, releasing volatile organic compounds (VOCs) which are excreted in the breath. Hence, breath VOC analysis may detect markers of rejection. Breath VOCs were analyzed in 28 heart transplant patients prior to endomyocardial biopsy. Ten volatile VOCs (including pentane) were significantly increased in those patients with histological evidence of rejection. A discriminant analysis model employing these VOCs identified rejection with 83.3% sensitivity and 100% specificity. The investigator hypothesizes that breath testing for VOCs may provide a useful new early diagnostic test for heart transplant rejection. The experimental plan will be to: 1) to perform breath VOC analysis in 200 cardiac transplant patients undergoing endomyocardial biopsy, 2) identify significant VOC markers of rejection and combine these in a model using discriminant analysis and 3) determine the sensitivity, specificity and predictive value of the breath test. The long term aim of the research is to determine if breath testing can detect heart transplant rejection and reduce the need for routine endomyocardial biopsy. PROPOSED COMMERCIAL APPLICATION: Potential commercial application not available.