SENSITIVE TEST OF GENERALIZED STEATORRHEA

目前脂肪泻定量评估的“黄金标准”， 在评估吸收不良时，普遍推荐72小时大便脂肪。 但却极大地未得到充分利用，因为它单调乏味，难以执行。 我们提出了一种新的方法来直接测量所有人的脂肪泻 消除了72小时大便测试的局限性的原因 保留了它的重要特征。我们的方法是基于管理 1，3-[13C]二硬脂基，2-油醇基甘油及其不可吸收标记物 用高脂肪的DyCl3餐，然后分析单人大便。这个 预计化验对脂溢性消化不良和肥胖症敏感 酸吸收不良，性价比高，并提供高度 与个体患者72小时大便脂肪呈正相关。在第一阶段，我们 证明了该方法的可行性，证明了Dy是 定量排泄，并遵循与13C相同的排泄动力学- 标有甘油三酯。在第二阶段，我们会研究 导致临床使用的实际测试的方法。我们将演示 标记的甘油三酯吸收是可重复性的，准确地反映 个别患者的脂肪泻程度，并有极好的 诊断灵敏度/特异度。开发的检测试剂盒和分析 服务将通过生物化学分析公司进行营销。 建议的商业应用： 这项研究将导致检测试剂盒和程序的可用 对于所有原因和要求的脂肪泻进行准确的定量 仅收集一张凳子。因为这一过程高度 重复性好、准确度高、使用相对简单，具有一定的适用性 在临床诊断和研究中使用。

The current "gold-standard" for quantitative assessment of steatorrhea, 72-h stool fat, is universally recommended in evaluation of malabsorption but vastly under-utilized because it is tedious and difficult to perform. We propose a novel approach for direct measurement of steatorrhea from all causes which eliminates the limitations of the 72-h stool test while retaining its important features. Our approach is based on administration of 1,3--[13C]distearyl, 2-oleolyl glycerol and the nonabsorbable marker DyCI3 with a high fat meal followed by analysis of a single stool. The test is expected to be sensitive to steatorrhea of maldigestion and fatty acid malabsorption, cost effective, and to provide results that are highly correlated with 72-h stool fat in individual patients. During Phase I, we demonstrated the feasibility of the approach by showing that Dy is quantitatively excreted and follows the same excretion kinetics as 13C- labeled triglyceride. During Phase II, we will study details of the approach leading to a practical test for clinical use. We will demonstrate that labeled triglyceride absorption is reproducible, accurately reflects the degree of steatorrhea in individual patients, and has excellent diagnostic sensitivity/specificity. The developed test kit and analytical services will be marketed through BioChemAnalysis Corporation. PROPOSED COMMERCIAL APPLICATION: This research will lead to the availability of a test kit and procedure for accurate quantitation of steatorrhea from all causes and requires collection of only a single stool. Because the procedure highly reproducible, accurate and relatively simple to use it will be applicable to clinical diagnostic use as well as use in research.